Controlled Genetic Manipulations by Alexei MorozovControlled Genetic Manipulations by Alexei Morozov

Controlled Genetic Manipulations

EditorAlexei Morozov

Hardcover | January 10, 2012

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The current demand for the development of techniques for controlled genetic manipulations is driven by the anatomical and physiological complexity of the brain and by the need for experimental models that can address this complexity through selective manipulation of defined components of the system: specific neuronal populations or selected synapses.  In Controlled Genetic Manipulations, expert researchers present detailed chapters to supply basic technical information about controlled genetic manipulations and to provide examples of creative implementation of this methodology when addressing a unique biological problem. Some chapters of the book describe the most recent developments in the basic methodology, which includes use of Cre-recombinase, methods for delivery of genetic material into the brain and the use of optogenetics, whereas other chapters focus on applying these techniques to addressing particular biological questions like structural and functional mapping of neuronal circuits, analysis of specific synaptic connections, modeling or gene therapy of neurological disorders.  As part of the Neuromethods series, chapters include key implementation advice that is crucial for getting optimal results. Authoritative and practical, Controlled Genetic Manipulations serves as a valuable guide to a variety of techniques, provided by many of the pioneers of the approaches.
Title:Controlled Genetic ManipulationsFormat:HardcoverDimensions:239 pagesPublished:January 10, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617795321

ISBN - 13:9781617795329

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Table of Contents

1. Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis            Jean-Paul Herman and Nicolas Jullien 2. Genetic Manipulations Using Cre and Mutant loxP Sites            Kimi Araki and Ken-ichi Yamamura 3. Using Recombinant Adeno-Associated Viral Vectors for Gene Expression in the Brain            Anke Van der Perren, Jaan Toelen, Jean-Marc Taymans, and Veerle Baekelandt 11. Analysis of Neuronal Circuits with Optogenetics 4. Using Lentiviral Vectors as Delivery Vehicles for Gene Therapy 11. Analysis of Neuronal Circuits with Optogenetics            Gregory A. Dissen, Jodi McBride, Alejandro Lomniczi, Valerie Matagne, Mauricio Dorfman, Tanaya L. Neff, Francesco Galimi, and Sergio R. Ojeda 5. Targeting Neurological Disease with siRNA 11. Analysis of Neuronal Circuits with Optogenetics            Jan Christoph Koch, Mathias Bähr, and Paul Lingor 6. In Utero Electroporation for Cellular Transgenesis in the Developing Mammalian Forebrain 11. Analysis of Neuronal Circuits with Optogenetics            Brady J. Maher and Joseph J. LoTurco 7. Single-Cell Electroporation of siRNA in Primary Neuronal Cultures 11. Analysis of Neuronal Circuits with Optogenetics            Masahiko Tanaka 8. Development and Application of Membrane-Tethered Toxins for Genetic Analyses of Neuronal Circuits 11. Analysis of Neuronal Circuits with Optogenetics            Sebastian Auer and Inés Ibañez-Tallon 9. Genetic Methods for Anatomical Analysis of Neuronal Circuits 11. Analysis of Neuronal Circuits with Optogenetics            Leah R. DeBlander, Aldis P. Weible, and Clifford G. Kentros 10. Functional Circuitry Analysis in Rodents Using Neurotoxins/Immunotoxins 11. Analysis of Neuronal Circuits with Optogenetics            Kazuto Kobayashi, Kana Okada, and Nobuyuki Kai            Feng Zhang, Le Cong, Garret D. Stuber, Antoine Adamantidis, and Karl Deisseroth 11. Analysis of Neuronal Circuits with Optogenetics            Feng Zhang, Le Cong, Garret D. Stuber, Antoine Adamantidis, and Karl Deisseroth