Correlative Light and Electron MIcroscopy by Paul VerkadeCorrelative Light and Electron MIcroscopy by Paul Verkade

Correlative Light and Electron MIcroscopy

byPaul Verkade, Thomas Muller-reichert, Thomas Muller-Reichert

Other | October 23, 2012

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The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

  • Covers many areas of CLEM by the best international scientists in the field
  • Includes a brief history and overview on CLEM methods
Dr. Paul Verkade's research focuses on the sorting mechanisms in intracellular transport pathways. His main tools are microscopy techniques, with an emphasis on electron microscopy (EM) in which field he has published over 50 papers. He has studied and got his PhD degree at the University of Utrecht, the Netherlands. After his post-doc...
Title:Correlative Light and Electron MIcroscopyFormat:OtherDimensions:460 pages, 1 × 1 × 1 inPublished:October 23, 2012Publisher:Elsevier ScienceLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0123914388

ISBN - 13:9780123914385


Table of Contents

    1. Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
    2. Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy
    3. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
    4. Correlative Light and Electron Microscopy in Parasite Research
    5. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
    6. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
    7. Correlative Light and Electron Microscopy of GFP
    8. Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
    9. Correlated Light Microscopy and Electron Microscopy (CLEM): Search... and Find!
    10. Capturing Endocytic Segregation Events with HPF-CLEM
    11. Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
    12. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the EarlyC. ElegansEmbryo
    13. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
    14. Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
    15. Visualizing Proteins in Electron Micrographs at Nanometer Resolution
    16. Atmospheric Scanning Electron Microscope for Correlative Microscopy
    17. Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
    18. Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms