Correlative Light and Electron MIcroscopy

Other | October 23, 2012

byThomas Mueller-Reichert, Thomas Mueller-reichert, Paul Verkade

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The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy;CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume ofMethods in Cell Biologycovers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly inC. elegansembryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.



  • Covers many areas of CLEM by the best international scientists in the field
  • Includes a brief history and overview on CLEM methods

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The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy;CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume ofMethods in Cell Biologycovers many areas of CLEM, including a...

Dr. Paul Verkade's research focuses on the sorting mechanisms in intracellular transport pathways. His main tools are microscopy techniques, with an emphasis on electron microscopy (EM) in which field he has published over 50 papers. He has studied and got his PhD degree at the University of Utrecht, the Netherlands. After his post-doc...

other books by Thomas Mueller-Reichert

Correlative Light and Electron Microscopy II
Correlative Light and Electron Microscopy II

Kobo ebook|Sep 1 2014

$172.79 online$224.41list price(save 23%)
Format:OtherDimensions:460 pages, 1 × 1 × 1 inPublished:October 23, 2012Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0123914388

ISBN - 13:9780123914385

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Table of Contents


    1. Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
    2. Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy
    3. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
    4. Correlative Light and Electron Microscopy in Parasite Research
    5. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
    6. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
    7. Correlative Light and Electron Microscopy of GFP
    8. Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
    9. Correlated Light Microscopy and Electron Microscopy (CLEM): Search... and Find!
    10. Capturing Endocytic Segregation Events with HPF-CLEM
    11. Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
    12. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the EarlyC. ElegansEmbryo
    13. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
    14. Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
    15. Visualizing Proteins in Electron Micrographs at Nanometer Resolution
    16. Atmospheric Scanning Electron Microscope for Correlative Microscopy
    17. Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
    18. Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms