Crystallization of Nucleic Acids and Proteins: A Practical Approach

Paperback | October 21, 1999

EditorArnaud Ducruix, Richard Giege

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Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucliec Acids and Proteins: A Practical Approach continues in the vein of the firstedition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology(maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films.As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminarycharacterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocolsto provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucliec Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology,crystallography, protein engineering, and biological chemistry.

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Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucliec Acids and Proteins: A Practical Approach continues in the vein of the firstedition by providing a detailed and rational guid...

Arnaud Ducruix is at CNRS. Richard Giege is at CNRS.
Format:PaperbackPublished:October 21, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199636788

ISBN - 13:9780199636785

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Table of Contents

PrefaceGiege and Ducruix: An introduction to the crystallogenesis of biological macromoleculesLorber and Giege: Biochemical aspects and handling of macromolecular solutions and crystalsBerne, Doublie and Carter, Jr: Molecular biology for structural biologyCarter, Jr: Experimental design, quantitive analysis, and the cartography of crystal growthDucruix and Giege: Methods of crystallizationRobert et al: Crystallization in gels and related methodsStura: Seeding techniquesDock-Bregeon, Moras and Giege: Nucleic acids and their complexesReiss-Husson and Picot: Crystallization of membrane proteinsRies-Kaut and Ducruix: From solution to crystals with a physico-chemical aspectVeesler and Boistelle: Diagnostic of prenucleation and nucleation by spectroscopic methods and background on the physicsBrisson, Lambert and Bergsma-Schutter: Two-dimensional crystallization of soluble proteins on planar lipid filmsStura and Gleichmann: Soaking techniquesSawyer and Turner: X-ray analysisIndex