Cytokine Protocols by Marc De LeyCytokine Protocols by Marc De Ley

Cytokine Protocols

EditorMarc De Ley

Paperback | November 10, 2010

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A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization.

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Title:Cytokine ProtocolsFormat:PaperbackDimensions:254 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372692

ISBN - 13:9781617372698

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Table of Contents

Large-Scale Generation of Plasmids that Express Type I InterferonPeter Härle and Daniel J. J. CarrIdentification of trans-Acting Factors by Electrophoretic Mobility Shift AssayRebecca J. Chan, Min You, and Gen-Sheng FengModulation of the Interferon-g Signal by Transfection of Cells with an Antisense-RNA Expressing VectorLeen Delrue and Marc De LeyCompetitive RT-PCR to Quantify Small Amounts of mRNAGabriela AustIn Situ Hybridization for Cytokines in Human Tissue BiopsiesEmily Mathey, John Pollard, and Patricia ArmatiPurification, Identification, and Synthesis of ChemokinesPatricia Menten, Anja Wuyts, and Jo Van DammeReceptor Isolation and Characterization: From Protein to GeneDaniela Novick and Menachem RubinsteinCrystallization of Cytokine-Receptor ComplexesWilliam J. Cook and Mark R. WalterBiosensor Analysis of Receptor-Ligand InteractionsSheng-Jiun Wu and Irwin ChaikenAnalysis of SH2 Ligands and Identification of Sites of InteractionManuel BacaAssays for Antiviral ActivityAnthony MeagerAssays for CytotoxicityAnthony MeagerAssays for ChemotaxisAnja Wuyts, Patricia Menten, Nancy Van Osselaer, and Jo Van DammeIn Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating FactorJames F. Eliason, Nadine Tare, Sharon Bowen, Tomoaki Inoue, and Ikuro HoriiDevelopment of a Mammalian Tet-On Expression Cell Line: Glucosylceramide Synthase Regulates TNF-a-Induced ApoptosisYong-Yu Liu and Myles C. CabotImmunohistochemical Detection of Cytokines in Human Tissue SectionsSandra Hulshof, Danny F. Dukers, Wim Vos, and Corline J. A. De GrootDetection of Cytokine- and Chemokine-Expressing Cells at the Single Cell LevelKarin Loré and Jan AnderssonDetection of Cytokine Signal Transduction "Cross-Talk" in Leukocyte ActivationHoward R. PettyAssay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine ProductionEtsushi Kuroda and Uki YamashitaIndex