Cytokines and Colony Stimulating Factors: Methods and Protocols by Dieter KörholzCytokines and Colony Stimulating Factors: Methods and Protocols by Dieter Körholz

Cytokines and Colony Stimulating Factors: Methods and Protocols

EditorDieter Körholz, Wieland Kiess

Paperback | November 10, 2010

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Hands-on laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Although the protocols require little previous experience, even highly skilled researchers will augment their skills with many novel timesaving techniques.
Title:Cytokines and Colony Stimulating Factors: Methods and ProtocolsFormat:PaperbackDimensions:496 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373184

ISBN - 13:9781617373183

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Table of Contents

Part I. IntroductionPrimary Immunodeficiencies Caused by Defects of Cytokines and Cytokine ReceptorsVolker WahnPart II. Detection Assays for Cytokines and Growth FactorsIntracellular Detection of T-Cell Cytokines: Differentiation of TH1 and TH2 CellsUrsula Banning and Dieter KörholzAnalysis of Cytokine Profiles in Human SkinJean Krutmann, Renz Mang, and Susanne Grether-BeckIntracytoplasmic Detection of Proinflammatory Cytokines and Chemokines in Monocytes by Flow CytometryChristian SchultzFlow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic CellsKerstin WillmannEvaluation of the Frequency of Virus-Specific CD8+ T Cells by Cytokine Flow CytometryBodo Hoffmeister, Felix Kiecker, Ingolf Surel, Elham Khatamzas, Volker Schuster, Hans-Dieter Volk, and Florian KernDetection of Common Cytokine and Colony Stimulating Factor Gene PolymorphismsThomas Lehrnbecher and Stephen J. ChanockApoptosis Induction by TRAILAngelika Eggert, Hauke Sieverts, and Henning WalczakAngiogenic Cytokines: Quantitative and Functional AnalysisAngelika Eggert and John H. MarisPart III. Protocols on Gene TherapyExperimental Strategies for Combined Suicide and Immune Cancer Gene Therapy: An OverviewChristof M. Kramm, Tim Niehues, and Nikolai G. RainovGene Therapy with Plasmids Encoding Cytokine- or Cytokine Receptor-IgG Chimeric ProteinsCiriaco A. Piccirillo and Gérald J. Prud'hommeInterferon-b Gene Therapy for the Treatment of ArthritisYuti Chernajovsky, Hanna Dreja, Kostas Triantaphyllopoulos, and David GouldGene Gun-Based In Vivo Gene Transfer: Application to DNA VaccinationTohru Sakai and Kunisuke HimenoPurification of the Eukaryotic Heat-Shock Proteins Hsp70 and gp96Arne von Bonin, Solveig H. Moré, and Minka BreloerGenetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-a MoietyJim Xiang and John R. GordonGenetic Engineering of Dendritic Cells by Adenovirus-Mediated TNF-a Gene TransferJim Xiang and Josh WuRetroviral Transfer of T-Cell Receptor Genes into Human Peripheral Blood LymphocytesTimothy M. Clay and Michael I. NishimuraGene Transfer to Articular Chondrocytes with Recombinant AdenovirusGlyn D. Palmer, Elvire Gouze, Jean-Noel Gouze, Oliver B. Betz, Christopher H. Evans, and Steven C. GhivizzaniGene Therapy of X-Linked Severe Combined ImmunodeficiencySalima Hacein-Bey-Abina, Genevieve de Saint Basile, and Marina Cavazzana-CalvoGene Transfer for Generation of Tumor and Leukemia VaccinesDagmar Dilloo and Andree ZibertCytokine Gene Delivery into the Central Nervous System Using Intrathecally Injected Nonreplicative Viral VectorsRoberto Furlan, Stefano Pluchino, Peggy C. Marconi, and Gianvito MartinoPart IV. Ex Vivo Cell ExpansionIn Vitro Regulation of Colony Stimulating Factor-Mediated Hematopoiesis in Healthy Individuals and Patients with Different Types of Myeloproliferative DiseaseThomas Vraetz, Peter D. Emanuel, and Charlotte M. NiemeyerIn Vitro Generation of Dendritic Cells from Cord Blood CD34+ Hematopoietic Progenitors CellsBruno Canque, Michele Rosenzwajg, and Jean Claude GluckmanGeneration of Transgenic T Cells from Human CD34+ Cord Blood CellsBruno Verhasselt, Evelien Naessens, and Veronique StoveEx Vivo Expansion of Umbilical Cord Blood Cells on Feeder LayersMartin BornhäuserEx Vivo Production of Cord Blood CD34+ Derived Myeloid Precursors After Serum-Free Static CultureSergio QuerolEx Vivo Expansion of Hematopoietic Stem CellsBeatriz Albella, José Carlos Segovia, Guillermo Guenechea, and Juan Antonio BuerenLarge-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical UsePhil Lefebvre and Isaac CohenCytokine-Mediated Expansion of Human NOD-SCID-Repopulating CellsKohichiro Tsuji, Takahiro Ueda, and Yasuhiro EbiharaPart V. Maturation and Differentiation of Dendritic CellsDifferentiation of Human Antigen-Presenting Dendritic Cells from CD34+ Hematopoietic Stem Cells In VitroXin-Sheng Ju and Martin ZenkeDendritic Cell Development from Mobilized Peripheral Blood CD34+ CellsDeanne M. R. Lathers, Nicholas Achille, and M. Rita I. YoungIn Vitro Maturation of Dendritic Cells from Blood Progenitors Lina Matera and Alessandra Galetto. Generation of Human Type 1- and Type 2-Polarized Dendritic Cells from Peripheral BloodPawel Kalinski, Pedro Vieira, Joost H. N. Schuitemaker, Quan Cai, and Martien KapsenbergPreparation of Human Dendritic Cells for Tumor VaccinationMichael R. ShurinGeneration of Leukemic Dendritic Cells from Patients with Acute Myeloid LeukemiaMohamad Mohty, Béatrice Gaugler, and Daniel OliveIndex