Cytotoxic Drug Resistance Mechanisms by Robert BrownCytotoxic Drug Resistance Mechanisms by Robert Brown

Cytotoxic Drug Resistance Mechanisms

EditorRobert Brown, Uta Böger-Brown

Paperback | November 9, 2010

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Leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The protocols contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Cytotoxic Drug Resistance Mechanisms illuminates all the clinically relevant mechanisms used as markers of the biological response to anticancer biotherapeutics today.
Title:Cytotoxic Drug Resistance MechanismsFormat:PaperbackDimensions:248 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617370932

ISBN - 13:9781617370939

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Table of Contents

Drug Resistance: The Clinical PerspectiveD. Alan Anthoney and Stanley B. KayeCell Sensitivity Assays: Clonogenic AssayJane A. PlumbCell Sensitivity Assays: The MTT AssayJane A. PlumbCell Sensitivity Assays: Detection of Apoptotic Cells In Vitro Using the TUNEL AssayNeil A. Jones and Caroline DiveAnalysis of Apoptosis in Tissue SectionsVicki Save, Philip J. Coates, and Peter A. HallMeasurement of P-glycoprotein FunctionHenk J. BroxtermanMeasuring MDR-1 by Quantitative RT-PCRSusan E. Bates, Zhirong Zhan, Joanna Regis, and Erick GamelinMicrotiter Plate Technique for the Measurement of Glutathione in Fresh and Cryopreserved Lymphoblasts Using the Enzyme Recycling MethodPamela R. Kearns and Andrew G. HallMeasurement of Reduced Glutathione Using High Pressure Liquid ChromatographyLinda A. Hogarth, Celia M. A. Rabello, and Andrew G. HallTopoisomerase I and II Activity AssaysPhilippe Pourquier, Glenda Kohlhagen, Li-Ming Ueng, and Yves Pommier5-Fluorouracil Metabolizing EnzymesHoward L. McLeod, Lesley H. Milne, and Stephen J. JohnstonMeasuring DNA Adducts by Immunoassay (ELISA)Michael J. TilbyMeasuring Drug-DNA Adducts in Individual CellsAdrian J. FrankMeasurement of Drug-Induced DNA Interstrand Crosslinking Using the Single Cell Electrophoresis (Comet) AssayVictoria J. Spanswick, Janet M. Hartley, Timothy H. Ward, and John A. HartleyPCR Analysis of Microsatellite InstabilityGillian L. HirstO 6-Alkylguanine-DNA Alkyltransferase AssayAmanda J. Watson and Geoffrey P. MargisonAnalysis of the p53 Status of Tumors: An Overview of MethodsJonas BerghBcl-2 Family ImmunohistochemistryLloyd R. Kelland and Philip J. BealeGenetic Analysis of Drug Resistance by Fluorescence In Situ HybridizationW. Nicol KeithGenetic Analysis of Drug Resistance by ReverseIn Situ HybridizationW. Nicol KeithIndex

Editorial Reviews

"This timely book will greatly benefit a wide array of individuals interested in cancer therapeutics, at both the clinical and basic science level. The editors and contributors did a good job of concisely presenting otherwise disparate methodologies in the context of a broad research field with the uniting purpose of understanding the biology underlying resistance to cancer chemotherapy. "-Doody's Health Sciences Book Review Journal"...the editors have managed to successfully compile a brief review of cytotoxic drug resistance mechanisms and an extensive manual of protocols for laboratory experimentation in this extremely important area of research. It is clear that any laboratory scientist in this field must be aware of the difficulty of translation to the clinic of anti-drug resistance lead compounds, and should keep a copy of this book within reach. Congratulations to the editors for assembling an important mix of laboratory and clinical scientists to create this volume."-The Quarterly Review of Biology"This book provides a comprehensive guide to a range of methods to a diverse range of drug resistance mechanisms including: Multidrug resistance (P-glycoprotein and MDR-1 gene analysis); cell sensitivity (clonogenic and MTT assays); Apoptosis (TUNEL, p 53 and Bcl-2); Glutathione-associated resistance (enzyme based glutathione assay and a HPLC method for reduced glutathione); DNA repair defects and damage (topoisomerase activity assays and DNA adduct measurements); 5-Fluorouracil metabolizing enzymes; Genetic analysis including microsatellite instability and fluorescence in situ hybridization....The methods are clearly described with each chapter consisting of an introduction and well described materials and methods sections. An extremely useful addition to most chapters was the "Notes" section where the respective authors suggest very useful hints, problem solving approaches and advice on the interpretation of results....the book is well written and useful addition to any laboratory investigating drug resistance in either clinical samples or tumor cell lines."-Cancer Forum"...a useful collection of protocols that can be used in the oncology laboratory for studies on drug resistance and molecular pharmacology....It should find its place on the library shelves of many laboratories of oncology."- Annals of Oncology