Dendritic Cell Protocols by Shalin H. NaikDendritic Cell Protocols by Shalin H. Naik

Dendritic Cell Protocols

EditorShalin H. Naik

Paperback | February 25, 2012

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Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular BiologyT series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field.
Title:Dendritic Cell ProtocolsFormat:PaperbackDimensions:464 pages, 10 × 7.01 × 0 inPublished:February 25, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161779676X

ISBN - 13:9781617796760

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Table of Contents

Part I: Reviews1. Review of Human DC subtypesXinsheng Ju, Georgina Clark, and Derek N.J. Hart2. Review of Murine Dendritic Cells: Types, Location, and DevelopmentTewfik Miloud, Günter J. Hämmerling, and Natalio GarbiPart II: Human DC Protocols3. Isolation of Human Blood DC SubtypesAndrew J. Kassianos, Sarah L. Jongbloed, Derek N.J. Hart, and Kristen J. Radford4. Isolation of Immature Primary Langerhans Cells from Human Epidermal SkinMarein A.W.P. de Jong, Lot de Witte, and Teunis B.H. Geijtenbeek5. Isolation and in vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells Remko Schotte, Heike Schmidlin, Maho Nagasawa, Wendy Dontje, Julien J. Karrich, Christel Uittenbogaart, Hergen Spits, and Bianca Blom6. In vivo Modulation of Gene Expression by Lentiviral Transduction in "Human Immune System" Rag2-/-gc-/- Mice Anja U. van Lent, Mireille Centlivre, Maho Nagasawa, Julien J. Karrich, Stephan Pouw, Kees Weijer, Hergen Spits, Bianca Blom, and Nicolas Legrand7. Generation of Stable Th1/CTL-, Th2- and Th17-inducing Human Dendritic Cells Pawel Kalinski, Eva Wieckowski, Ravikumar Muthuswamy, and Esther de Jong8. Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in TransplantationHeth R. Turnquist, Ryan Fischer, and Angus W. Thomson9. Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell VaccinesMichael Erdmann and Beatrice Schuler-ThurnerPart III: Murine Dendritic Cell Methods10. Generation of Mouse CD8+, CD8-, and Plasmacytoid Dendritic Cells Equivalents in vitro Using flt3 LigandShalin H. Naik, Meredith O'Keeffe, Anna Proietto, Hubertus Hochrein, Ken Shortman, and Li Wu11. Generation of Large Numbers of pro-DCs and pre-DCs in vitroShalin H. Naik12. The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSFMeredith O'Keeffe, Ben Fancke, and Hubertus Hochrein13. Isolation of Common Dendritic Cell Progenitor (CDP) from Mouse Bone MarrowNobuyuki Onai, Markus G. Manz, and Michael A. Schmid14. The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow CytometryDavid Vremec15. Isolation of Cutaneous Dendritic Cells Julie Helft and Miriam Merad16. Isolation of Skin Dendritic Cells from Mouse and ManPatrizia Stoitzner, Nikolaus Romani, Alexander D. McLellan, Christoph H. Tripp, and Susanne Ebner17. Identification and Isolation of Rodent Respiratory Tract Dendritic Cells Philip A. Stumbles, Deborah H. Strickland, Mathew E. Wikstrom, Jennifer A. Thomas, Christophe von Garnier, and Patrick G. Holt18. Isolation of Mouse Thymic Dendritic Cell PrecursorsLi Wu and Angela D'Amico19. Isolation of Rat Intestinal Lymph DCSimon Milling and Gordon MacPherson20. Mouse Models of Viral Infection: Influenza Infection in the LungAdele M. Mount and Gabrielle T. Belz21. DCs in Mouse Models of Intracellular Bacterial InfectionMichael Neuenhahn, Matthias Schiemann, and Dirk H. Busch22. Studying the Function of Dendritic Cells in Mouse Models of AsthmaPhilippe Pouliot, Monique A. Willart, Hamida Hammad, and Bart N. Lambrecht23. Direct ex vivo Activation of T cells for Analysis of Dendritic Cell Antigen PresentationGabrielle T. Belz24. Methods to Study Pulmonary Dendritic Cell Migration Claudia Jakubzick and Gwendalyn J. Randolph25. Measuring pH, ROS Production, Maturation, and Degradation in Dendritic Cell Phagosomes Using Cytofluorometry-Based AssaysAriel Savina, Pablo Vargas, Pierre Guermonprez, Ana-Maria Lennon, and Sebastian Amigorena26. Adaptive Treg Generation by DCs and Their Functional AnalysisLi Wang27. Generation of Parabiotic Mice for the Study of DC and DC Precursor CirculationClaudia Waskow28. Defining in vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice Liat Bar-On and Steffen Jung

Editorial Reviews

From the reviews of the second edition:"Written for principal investigators and their students, postdocs, and staff, providing very practical step-by-step instructions on how to work with DC. . Overall, this book provides a nice compilation of methods for studying dendritic cells. The book will be particularly useful for those studying murine DC, but will also provide some useful . information for those initiating human DC work. . this updated book, which is unique in the field, will make a nice addition to personal and institutional biomedical research libraries." (Patricia Fitzgerald-Bocarsly, Doody's Review Service, July, 2010)