Developmental Hematopoiesis: Methods and Protocols by Margaret H. BaronDevelopmental Hematopoiesis: Methods and Protocols by Margaret H. Baron

Developmental Hematopoiesis: Methods and Protocols

EditorMargaret H. Baron

Paperback | November 10, 2010

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The first collection of methods to study embryonic and fetal hematopoiesis in both invertebrate (fruit flies) and vertebrate (frog, fish, mouse, chick, and human) organisms. These state-of-the-art techniques range from the genetic, molecular, and cellular, to cell and embryo explant culture and whole animals, including in vivo imaging. Bioinformatic and functional genomic approaches for studying stem cells and their supportive stromal cells are also discussed. The protocols follow the successful Methods in Molecular MedicineT series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Title:Developmental Hematopoiesis: Methods and ProtocolsFormat:PaperbackDimensions:480 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617374784

ISBN - 13:9781617374784

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Table of Contents

Part I. Genetic ApproachesGene-Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the MouseHanna K. A. Mikkola and Stuart H. OrkinInducible Transgene Expression in Mouse ES CellsDavid T. Ting, Michael Kyba, and George Q. DaleyQuantitative Trait Analysis in the Investigation of Function and Aging in Hematopoietic Stem CellsHans-Willem SnoeckPart II. Analysis of Cell Fate Outcomes in Transplantation ModelsFlow Cytometric Analysis of Hematopoietic DevelopmentEdward F. Srour and Mervin C. YoderReconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem CellsElisabeth H. Javazon, Aziz M. Merchant, Enrico Danzer, and Alan W. FlakeReconstitution of Hematopoiesis Following Transplantation Into Neonatal MiceScott A. Johnson and Mervin C. YoderPart III. Animal ModelsDrosophilaHemocyte Development During Drosophila EmbryogenesisRobert A. Schulz and Nancy FossettXenopusTracking and Programming Early Hematopoietic Cells in Xenopus EmbryosMaggie Walmsley, Aldo Ciau-Uitz, and Roger PatientFate Mapping Hematopoietic Lineages in the Xenopus EmbryoMary Constance Lane and Michael D. SheetsAnalyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain)Yumi Izutsu and Mitsugu MaénoUse of Flow Cytometry and Combined DNA/Surface Staining for Analysis of Hematopoietic Development in the Xenopus EmbryoJames B. TurpenZebrafishAnalysis of Hematopoietic Development in the ZebrafishNoëlle N. Paffett-Lugassy and Leonard I. ZonImaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish EmbryosPhilippe Herbomel and Jean-Pierre Levraud. ChickIn Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian EmbryoFrançoise Dieterlen-Lièvre, Sophie Creuzet, and Josselyne SalaünMouseMouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular DevelopmentMargaret H. Baron and Deanna MohnHematopoietic Stem Cell Enrichment From the AGM Region of the Mouse EmbryoCatherine Robin and Elaine DzierzakHematopoietic Stem Cell Development During Mouse EmbryogenesisJulien Y. Bertrand, Sebastien Giroux, Ana Cumano, and Isabelle GodinAnalysis of Hematopoietic Progenitors in the Mouse EmbryoJames Palis and Anne KoniskiIn Vivo and In Vitro Assays of Thymic OrganogenesisJulie Gordon, Valerie A. Wilson, Billie A. Moore-Scott, Nancy R. Manley, and C. Clare BlackburnRetroviral Transduction in Fetal Thymic Organ CultureBronwyn M. Owens, Robert G. Hawley, and Lisa M. SpainExpansion and Differentiation of Immature Mouse and Human Hematopoietic ProgenitorsHelmut Dolznig, Andrea Kolbus, Cornelia Leberbauer, Uwe Schmidt, Eva-Maria Deiner, Ernst W. Müllner, and Hartmut BeugA New Clonal Assay System for Lymphoid and Myeloid LineagesHiroshi Kawamoto and Yoshimoto KatsuraHematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in CultureKyunghee Choi, Yun Shin Chung, and Weh Jie ZhangAnalysis of the Vascular Potential of Hematopoietic Stem CellsSteven M. Guthrie, Sergio Caballero, Robert N. Mames, Maria B. Grant, and Edward W. ScottDynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo CultureElizabeth A.V. Jones, Margaret H. Baron, Scott E. Fraser, and Mary E. DickinsonFluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic DifferentiationMatthias Stadtfeld, Florencio Varas, and Thomas GrafHumanAnalysis of Hematopoietic Development During Human Embryonic OntogenesisManuela Tavian and Bruno PéaultHematopoietic Development of Human Embryonic Stem Cells in CultureXinghui Tian and Dan S. KaufmanPart IV. Bioinformatics and Functional GenomicsA Functional Genomics Approach to Hematopoietic Stem Cell RegulationJason A. Hackney and Kateri A. MooreIndex