Diabetes Mellitus by Sabire ÖzcanDiabetes Mellitus by Sabire Özcan

Diabetes Mellitus

EditorSabire Özcan

Paperback | November 10, 2010

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Leading researchers and clinical investigators describe their best cutting-edge techniques for studying, at both the molecular and biochemical levels, the defects in insulin production and action associated with diabetes. Written in step-by-step detail to ensure ready reproducibility and robust results, these techniques allow investigators, both novices and those already active in the field, to study every major facet of insulin production and action. Each protocol includes an introduction to the technique, an explanation of its application, and a list of materials. Practical notes discuss how to avoid pitfalls, as well as how to adapt the methods to your own research.
Title:Diabetes MellitusFormat:PaperbackDimensions:208 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373885

ISBN - 13:9781617373886

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Table of Contents

Part I. Insulin ProductionIsolation of Islets of Langerhans from Rodent PancreasColleen B. Kelly, Libby A. Blair, John A. Corbett, and Anna L. ScarimPurification of Rat Pancreatic b-Cells by Fluorescence-Activated Cell SortingGeert Stangé, Mark Van De Casteele, and Harry HeimbergAssessment of Insulin Secretion in the MouseMarcela Brissova, Wendell E. Nicholson, Masakazu Shiota, and Alvin C. PowersDetection of Insulin Production by ImmunohistochemistrySusan C. Campbell and Wendy M. MacfarlaneQuantification of the Level of Insulin Gene ExpressionSusan C. Campbell and Wendy M. MacfarlaneChromatin Immunoprecipitation Using Isolated Islets of LangerhansMarcelina Párrizas, Sylvia F. Boj, Reini F. Luco, Miguel Angel Maestro, and Jorge FerrerAdenoviral Gene Transfer into b-Cell LinesAmber L. Mosley and Sabire ÖzcanUtilization of NOD Mice in the Study of Type 1 DiabetesDennis G. Karounos and Susan E. GoesPart II. Insulin ActionIntroduction of DNA into 3T3-L1 Adipocytes by ElectroporationShuichi Okada, Masatomo Mori, and Jeffrey E. PessinAnalysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 AdipocytesJaganathan Lakshmanan, Jeffrey S. Elmendorf, and Sabire ÖzcanFractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 AdipocytesJeffrey S. ElmendorfVisualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet AssayJohn B. Knight and Ann Louise OlsonAssaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor SubstratesEileen L. Whiteman and Morris J. BirnbaumMeasuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase ActivityLi-Ping Wang and Scott A. SummersAssaying AKT/ Protein Kinase B ActivityKyle L. Hoehn and Scott A. SummersMeasurements of Cellular Phosphoinositide Levels in 3T3-L1 AdipocytesDaryll B. DeWaldMeasurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 AdipocytesMatthew J. BradyMeasurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal MuscleJoseph T. Brozinick, Jr.Single Embryo Measurement of Basal- and Insulin-Stimulated Glucose UptakeMaggie M-Y. Chi and Kelle H. MoleyImmunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose TransportersMary O. Carayannopoulos and Kelle H. MoleyIndex

Editorial Reviews

"This book provides all the critical tools needed for the productive study of diabetes mellitus today." - Clinical Laboratory International"Each chapter/protocol is well referenced and the book is indexed. Because Diabetes Mellitus: Methods and Protocols provides all the critical tools needed for the productive study of diabetes mellitus, it is a necessary purchase for libraries supporting basic research programs that study the molecular and biochemical bases of diabetes mellitus." -E-STREAMS