Differential Display: A Practical Approach

Paperback | May 1, 2000

EditorRon A. Leslie, Harry Robertson

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One of the most challenging tasks facing the modern biological research laboratory is to make sense of the enormous amount of data being generated by various genome projects currently underway, and especially the human genome project. Understanding the ways in which genes are differentiallyexpressed in various tissues and cell types, throughout ontogenetic development and in pathological processes, will go a long way towards understanding the function of all these 'new' genes and their protein products. Differential Display explains in detail how to perform the technique of RT-PCRDifferential Display in various kinds of experimental biological systems. It also examines this technique in the context of other methods of studying differential gene expression such as subtractive hybridisation and the use of high-density gene microarrays combined with hybridisation techniquesand automatic image analysis.

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One of the most challenging tasks facing the modern biological research laboratory is to make sense of the enormous amount of data being generated by various genome projects currently underway, and especially the human genome project. Understanding the ways in which genes are differentiallyexpressed in various tissues and cell types, ...

Ron A. Leslie is at SmithKline Beecham Pharmaceuticals, Harlow. Harry A. Robertson is at Dalhousie University, Halifax.

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Format:PaperbackPublished:May 1, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:019963758X

ISBN - 13:9780199637584

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Table of Contents

An introduction to differential display and related techniquesRecent advances in fluorescent differential displayPractical aspects of the experimental design for differential display of transcripts obtained from complex tissuesRAP-array : expression profiling using reduced complexity probes for cDNA arraysThe use of RT-PCR differential display in single-celled organisms and plant tissuesA modified approach for the efficient display of 3' - end restriction fragments of cDNAsCloning of differentially expressed brain cDNAsUse of suppression subtractive hybridization strategy to identify differential gene expression in brain ischemic toleranceStudying gene expression profiles in specialized brain regions by microsageConstruction and applications of gene microarrays on nylon membranesSubtracted differential display and antisense approaches to analyzing brain signallingDifferential display analysis of memory-associated genes