DNA Cloning 1: A Practical Approach: Core Techniques

Paperback | March 1, 1992

EditorD. M. Glover, B. D. Hames

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This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomiclibraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date account of each topic by established researchers. These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

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This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomiclibraries, probe preparation, analysis of gene org...

D.M. Glover is at University of Dundee. B.D. Hames is at University of Leeds.

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Format:PaperbackDimensions:288 pages, 9.21 × 6.14 × 0.63 inPublished:March 1, 1992Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199634769

ISBN - 13:9780199634767

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Table of Contents

1. Techniques for transformation of E.coli2. Lambda replacement vectors in the construction of genomic DNA libraries3. Procedures for cDNA cloning4. Making probes5. Using cloned DNAs to analyse gene organization and expression6. Choices of single-stranded vectors and their application for in vitro mutagenesis7. PCR and its applications8. DNA sequencing