DNA Cloning 2: A Practical Approach: Expression Systems

Paperback | March 1, 1992

EditorD. M. Glover, B. D. Hames

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Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols,troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecularbiology laboratories.

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Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols,troubleshooting, and details of the latest appli...

B. D. Hames is at University of Leeds. D. Glover is at University of Dundee.

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Format:PaperbackDimensions:274 pages, 9.21 × 6.14 × 0.59 inPublished:March 1, 1992Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199634785

ISBN - 13:9780199634781

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Table of Contents

1. Screening expression libraries with antibody and protein probes2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies3. Purification of over-produced proteins from E.coli cells4. Production of monoclonal antibodies against proteins expressed in E.coli5. Expression of cloned genes in yeast6. Intraction trap cloning with yeast7. The baculovirus expression system

Editorial Reviews

`The present book consists of 7 chapters that each coprehensively describe different aspects of the subject and which may be read independently ... this book is very detailed and informative and contains much information with key literature citations of use for any scientist working in thearea of heterologous protein expression ... The book can thus be highly recommended.'Bent Honore, FEBS Letters 393 (1996)