DNA Cloning 4: A Practical Approach: Mammalian Systems by D. M. GloverDNA Cloning 4: A Practical Approach: Mammalian Systems by D. M. Glover

DNA Cloning 4: A Practical Approach: Mammalian Systems

EditorD. M. Glover, B. D. Hames

Paperback | April 30, 1999

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DNA cloning techniques have made their impact to most areas of biological research. This is the fourth and last volume in this set, offering a complete update and extension into new areas of David Glover's original DNA Cloning volumes. This volume describes a variety of techniques for geneticmanipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area byestablished researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments.This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.
D. Glover is at University of Dundee. D. B. Hames is at University of Leeds.
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Title:DNA Cloning 4: A Practical Approach: Mammalian SystemsFormat:PaperbackDimensions:340 pages, 9.21 × 6.14 × 0.67 inPublished:April 30, 1999Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199634807

ISBN - 13:9780199634804

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Table of Contents

1. High efficiency gene transfer into mammalian cells2. Construction and characterization of Vaccinia virus recombinants3. Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells4. Retroviral vectors5. Genetic manipulation of embryonic stem cells6. Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs7. Genomic and expression analysis of transgenic animals8. Expression using a defective Herpes simplex virus (HSV-1) vector system9. Adenovirus vectors