DNA cloning techniques have made their impact to most areas of biological research. This is the fourth and last volume in this set, offering a complete update and extension into new areas of David Glover's original DNA Cloning volumes. This volume describes a variety of techniques for geneticmanipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area byestablished researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments.This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.