DNA Electrophoresis: Methods and Protocols by Svetlana MakovetsDNA Electrophoresis: Methods and Protocols by Svetlana Makovets

DNA Electrophoresis: Methods and Protocols

EditorSvetlana Makovets

Hardcover | August 5, 2013

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In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.
Title:DNA Electrophoresis: Methods and ProtocolsFormat:HardcoverDimensions:265 pages, 10 × 7.01 × 0.01 inPublished:August 5, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627035648

ISBN - 13:9781627035644

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Table of Contents

1. DNA Electrophoresis: Historical and Theoretical Perspectives Gareth A. Roberts and  David T.F. Dryden 2. Basic DNA Electrophoresis in Molecular Cloning: a Comprehensive Guide for BeginnersSvetlana Makovets 3. Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-dimensional (2D) Native-native Agarose Gel ElectrophoresisNicholas P. Robinson 4. Analysis of DNA Structures from Eukaryotic cells by Two-dimensional Native-native DNA Agarose Gel ElectrophoresisAndreas S. Ivessa 5. Directionality of Replication Fork Movement Determined by Two-dimensional Native-native DNA Agarose Gel ElectrophoresisAndreas S. Ivessa 6. Native/Denaturing Two-Dimensional DNA Electrophoresis And Its Application To The Analysis Of Recombination IntermediatesJessica Lao, Shangming Tang and Neil Hunter 7. Plasmid DNA Topology Assayed by Two-dimensional Agarose Gel ElectrophoresisJorge B. Schvartzman, María-Luisa Martínez-Robles, Pablo Hernández, and Dora B. Krimer 8. A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-Quantitation of DNA Single-Strand BreaksBrian Pachkowski and Jun Nakamura 9. Denaturing Gradient Gel Electrophoresis (DGGE)Fiona Strathdee and Andrew Free 10. Polyacrylamide Temperature Gradient Gel ElectrophoresisViktor Viglasky  11. Separation of DNA Oligonucleotides using Denaturing Urea-PAGEFiona Flett and Heidrun Interthal 12. Pulsed-field Gel Electrophoresis of Bacterial Chromosomes Julia S. P. Mawer and David R. F. Leach 13. Resolution of Budding Yeast Chromosomes using Pulse Field Gel ElectrophoresisAziz El Hage  and Jonathan Houseley 14. Analysis of DNA Damage Via Single Cell ElectrophoresisDiana Anderson and Julian Laubenthal15. Fluorescence in situ Hybridisation on Electrophoresed Cells to Detect Sequence Specific DNA DamageJulian Laubenthal and Diana Anderson  16. Analysis of Protein-DNA Interactions using PAGE: Band-shift AssaysLynn Powell 17. Assaying Cooperativity of Protein-DNA Interactions using Agarose Gel ElectrophoresisTanya L. Williams and Daniel L. Levy 18. DNA Bending by Proteins: Utilizing Plasmid pBendAT as a ToolFenfei Leng 19. Using PCR coupled to PAGE for Detection and Semi-quantitative Evaluation of Telomerase ActivityLaura Gardano