DNA Electrophoresis Protocols for Forensic Genetics by Antonio AlonsoDNA Electrophoresis Protocols for Forensic Genetics by Antonio Alonso

DNA Electrophoresis Protocols for Forensic Genetics

EditorAntonio Alonso

Hardcover | December 3, 2011

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Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of  PCR amplicons obtained from DNA genomic markers with different  inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It  includes protocols for  profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and  mtDNA  hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful  Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Title:DNA Electrophoresis Protocols for Forensic GeneticsFormat:HardcoverDimensions:407 pagesPublished:December 3, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617794600

ISBN - 13:9781617794605

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Table of Contents

Part I: Current Protocols on Capillary Electrophoresis of Amplified DNA Fragments    1.         An Overview of  DNA Typing Methods for Human Identification: Past, Present, and FutureRobyn Thompson, Silvia Zoppis, and Bruce McCord  2.         Capillary Electrophoresis and 5-channel LIF Detection of a 26plex Autosomal STR Assay for Human IdentificationCarolyn R. Hill.  3.         Capillary Electrophoresis of Mini-STR Markers to Genotype Highly Degraded DNA SamplesMichael Coble 4.         Interpretation Guidelines of a Standard Y-chromosome STR 17-plex PCR-CE Assay for Crime CaseworkLutz Roewer and Maria Geppert 5.         Capillary Electrophoresis of an X-Chromosome STR Decaplex for Kinship Deficiency CasesLeonor Gusmão, Cíntia Alves, Iva Gomes, and Paula Sanchéz-Diz 6.         A 48-plex autosomal SNP GenPlex Assay for Human Individualization and Relationship TestingCarmen Tomas, Claus Børsting, and Niels Morling.  7.         Typing of 49 Autosomal SNPs by Single Base Extention and Capillary Electrophoresis for Forensic Genetic TestingClaus Børsting, Carmen Tomas, and Niels Morling  8.         A 34-plex Autosomal SNP Single Base Extension Assay for Ancestry InvestigationsChristopher Phillips, Manuel Fondevila, and Maviki Lareu 9.         SNaPshot® Minisequencing Analysis of Multiple Ancestry-informative Y-SNPs    Using Capillary ElectrophoresisMaria Geppert and Lutz Roewer 10.       Capillary Electrophoresis of 38 Non-coding Biallelic Mini-Indels for Degraded Samples and as Complementary Tool in Paternity TestingRui Pereira and Leonor Gusmão 11.       Capillary Electrophoresis of an 11-plex mtDNA Coding Region SNP Single Base Extension Assay for Discrimination of the most Common Caucasian HV1/HV2 MitotypePeter M. Vallone 12.       Capillary Electrophoresis of a Multiplex Reverse Transcription-Polymerase Chain Reaction to Target Messenger RNA Markers for Body Fluid IdentificationCordula Haas, Erin Hanson, and  Jack Ballantyne  13.       DNA Extraction from Aged Skeletal Samples for STR Typing by Capillary ElectrophoresisRene Huel, Sylvain Amory, Ana Bilic, Stojko Vidovic, Edin Jasaragic, and Thomas Parsons 14.       Interpretation Guidelines for Multilocus STR Forensic Profiles from Low Template DNA SamplesZoran M. Budimlija and Theresa Caragine 15.       Interpretation Guidelines of Mixed STR Multilocus Electrophoretic Profiles Juan Antonio Luque   16.       Capillary Electrophoresis Analysis of a 9-plex STR Assay for Canine Genotyping Barbara van Asch and Antonio Amorim 17.       Capillary Electrophoresis of DNA from Cannabis sativa for Correlation of Samples to Geographic OriginHeather Miller Coyle 18.       Capillary Electrophoresis of Multigene Barcoding Chloroplast Markers for Species Identification of Botanical Trace EvidenceGianmarco Ferri, Beatrice Corradini, and Milena Alu  Part II: Capillary electrophoresis and DNA Sequencing  19.       Capillary Electrophoresis of Big-Dye Terminator Sequencing Reactions for Human mtDNA Control Region Haplotyping in the Identification of Human RemainsMarta Montesino & Lourdes Prieto 20.       Capillary Electrophoresis of Human mtDNA Control Region Sequences from Highly Degraded Samples using Short mtDNA Amplicons Odile Loreille and  Jodi A. Irwin  21.       INTERPRETATION GUIDELINES OF MTDNA CONTROL REGION SEQUENCE ELECTROPHEROGRAMS IN FORENSIC GENETICSManuel Crespillo 22.       Capillary Electrophoresis of mtDNA Cytochrome b Gene Sequences for Animal Species IdentificationAdrian Linacre  23.       Sequence Analysis of the Canine Mitochondrial DNA Control Region from Shed Hair Samples in Criminal InvestigationsCordula  Berger, B. Berger and Walther Parson  Part III: Advances in Microchip Capillary Electrophoresis 24.       Integrated Sample Cleanup and Microchip Capillary Array Electrophoresis for High-Performance Forensic STR ProfilingPeng Liu, Susan A. Greenspoon, Stephanie H. I. Yeung, and Richard A. Mathies 25.       Microchip Capillary Electrophoresis Protocol to Evaluate Quality and Quantity of mtDNA Amplified Fragments for DNA Sequencing in Forensic GeneticsCoro Fernandez and  Antonio Alonso.  26.       Microchip Capillary Electrophoresis of Multi Locus VNTR Analysis for Genotyping of Bacillus Anthracis and Yersinia Pestis in Microbial Forensic CasesAndrea Ciammaruconi