DNA-Protein Interactions: A Practical Approach

Paperback | July 1, 2000

EditorAndrew Travers, Malcolm Buckle

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DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecularregulatory interactions involved. This book covers all the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA, with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatinstructure. Knowledge of basic techniques is assumed, although advances in fundamental fields are covered.

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DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecularregulatory interactions involved. This book cov...

Andrew Travers is at MRC Laboratory of Molecular Biology, Cambridge. Malcolm Buckle is at MRC Laboratory of Molecular Biology, Cambridge.
Format:PaperbackDimensions:398 pages, 9.69 × 7.44 × 0.91 inPublished:July 1, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199636915

ISBN - 13:9780199636914

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Table of Contents

Expression SystemsGel electrophoresis and bending in cisplatin-modified linear DNAUse of DNA microcircles in protein-DNA binding studiesUse of topology to measure protein-induced DNA bend and unwinding anglesBandshift, gel retardation, or electrophoretic mobility shift assaysPractical aspects of Fluorescence Resonance Energy Transfer (FRET) and its applications in nucleic acid biochemistryDetermination of DNA-ligand interactions by fluorescence correlation spectroscopyDNA wrapping in E. Coli RNA polymerase open promotor complexes revealed by scanning force microscopyMicrocalorimetry of protein-DNA interactionsDNA-protein cross-linking by formaldehyde: a probe for RNAP-open promoter complex structureSolid-phase DNAse I footprintingHydroxyl radical footprintingRadiolytic cleavage of DNA: Mapping of the Protein Interaction SitesUV - Laser photoreactivity of nucleoprotein complexes in vitroIn vivo UV-laser footprintingDigitization and quantitative analysis of footprinting gelsMapping histone positions in chromatin by protein-directed DNA cross-linking and cleavageKinetics of DNA-protein interactionsKinetics of DNA interactions studied by surface plasmon resonance spectroscopyQuantitative DNase I Kinetics FootprintingReal time fluorescence anisotropy for studying protein-DNA interactionsAnalysis of protein-DNA interactions in complex nucleoprotein assembliesSite-specific protein-DNA photocrosslinkingVisualization of protein-DNA complexes by cryo em and on gridsCharacterization of T7 RNA polymerase protein-DNA interactions during the initiation and elongation phases