DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs by Neil OsheroffDNA Topoisomerase Protocols: Volume II: Enzymology and Drugs by Neil Osheroff

DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs

EditorNeil Osheroff, Mary-Ann Bjornsti

Paperback | November 10, 2010

Pricing and Purchase Info

$204.18 online 
$219.95 list price save 7%
Earn 1021 plum® points
Quantity:

In stock online

Ships free on orders over $25

Not available in stores

about

An unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatenation, and ATP hydrolysis. Several modified DNA substrates, used to dissect enzyme mechanisms by trapping intermediates, are also described. Methodologies to determine the action of topoisomerase-targeted drugs include biochemical assays of drug-induced enzyme-DNA complexes, methods for assaying drug uptake, and cell-based assays for determining the specificity and mechanisms of drug resistance. A companion volume, DNA Topoisomerase Protocols, I: DNA Topology and Enzymes, provides state-of-the-art experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function.
Title:DNA Topoisomerase Protocols: Volume II: Enzymology and DrugsFormat:PaperbackDimensions:350 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617370576

ISBN - 13:9781617370571

Look for similar items by category:

Reviews

Table of Contents

Assaying DNA Topoisomerase I Relaxation ActivityLance Stewart and James J. ChampouxDNA Topoisomerase II-Catalyzed DNA DecatenationAndrea Haldane and Daniel M. SullivanPlasmid DNA Supercoiling by DNA GyrasePenny J. Sayer, Martin L. Goble, Mark Oram, and L. Mark FisherAnalyzing Reverse Gyrase ActivityMichel Duguet, Christine Jaxel, Anne-Céile Déclais, Fabrice Confalonieri, Janine Marsault, Claire Bouthier de la Tour, Marc Nadal, and Christiane PortemerTopoisomerase II-Catalyzed ATP Hydrolosis as Monitored by Thin-Layer ChromatographyPaul S. Kingma, John M. Fortune, and Neil OsheroffUse of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase IIJanet E. LindsleyAnalysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift AssayStewart ShumanFilter Binding Assays for Topoisomerase-DNA ComplexesJoaquim RocaDNA Topoisomerase I-Mediated Nicking of Circular Duplex DNAJames J. ChampouxAnalysis of Cleavage Complexes Using Reactive Inhibitor DerivativesKenneth N. Kreuzer, Catherine H. Freudenreich, and Yves PommierUncoupling of Topoisomerase-Mediated DNA Cleavage and ReligationAnni H. Anderson, Kent Christiansen, and Ole WestergaardSynthesis and Use of DNA Containing a 5'-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA TopoisomerasesAlex B. Burgin, JrIsolation of Covalent Enzyme-DNA ComplexesThomas C. Rowe, Dale Grabowski, and Ram GanapathiICE Bioassay: Isolating In Vivo Complexes of Enzyme to DNADeepa Subramanian, Christine Sommer Furbee, and Mark T. MullerDNA Unwinding Test Using Eukaryotic DNA Topoisomerase ILinus L. ShenDrug-DNA InteractionsDavid E. GravesQuinolone Interactions with DNA and DNA GyraseLinus L. ShenBactericidal Assays for FluoroquinolonesThomas D. GootzDrug Toxicity in E. coli Cells Expressing Human DNA Topoisomerase IScott T. Taylor and Rolf MenzelDrug-Induced Cytotoxicity in Tissue CultureCynthia E. Herzog and Leonard A. ZwellingAnalysis of Drug Uptake-Flow CytometryGrace Zoorob and Thomas BurkeCell Cycle Analysis of Drug-Treated CellsFrank Traganos, Gloria Juan, and Zbigniew DarzynkiewiczAssaying Drug-Induced ApoptosisZbigniew Darzynkiewicz, Gloria Juan, and Frank TraganosSingle-Strand Conformational Polymorphism Analysis of DNA Topoisomerases I and IIMary K. DanksPCR-Based Cloning of DNA Topoisomerase GenesWai Mun HuangTopoisomerase II-Catalyzed Relaxation and Catenation of Plasmid DNAJohn M. Fortune and Neil OsheroffTopoisomerase II-Mediated Cleavage of Plasmid DNAD. Andrew Burden, Stacie J. Froelich-Ammon, and Neil OsheroffDrug-induced Stabilization of Covalent DNA Topoisomerase I-DNA Intermediates: DNA Cleavage AssaysPaola Fiorani, Christine L. Hann, Piero Benedetti, and Mary-Ann BjornstiStudying DNA Topoisomerase I-Targeted Drugs in the Yeast Saccharomyces cerevisiaeMichael H. Woo, John R. Vance, and Mary-Ann BjornstiYeast Systems for Demonstrating the Transfer of Antitopoisomerase II AgentsJohn L. Nitiss and Karen C. NitissIndex

Editorial Reviews

"This book completes a comprehensive collection of assays, including the weird and wonderful, that can be used to investigate the enzymology and drug inhibition of topoisomerases...I recommend any students about to undertake a PhD degree in this area to buy a copy. Even those who are well versed in the biology of topoisomerases should consider buying both volumes of this book, they will surely find some things they didn't know - I did!" - MicroBiology Today"Described by expert experimentalists who have perfected the techniques, these reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatentaion, and ATP hydrolysis." - Anticancer Research"An unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs." -Tumori