DNA Vaccines by Mark W. SaltzmanDNA Vaccines by Mark W. Saltzman

DNA Vaccines

EditorMark W. Saltzman, Hong Shen, Janet L. Brandsma

Paperback | November 10, 2010

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In this completely revised and updated second edition of DNA Vaccines: Methods and Protocols, W. Mark Saltzman presents a comprehensive collection of DNA vaccine protocols, written by leading experts in the groundbreaking field of DNA vaccination. Divided into five sections, this volume contains state-of-the-art and practical procedures on the latest DNA vaccine technology. Part I contains DNA vaccine design protocols, focusing on methods that achieve optimal expression in host cells. Part II is dedicated to presenting methods for DNA delivery, and covers both the range of administration methods available for vaccine administration and a variety of techniques for improving the efficiency of delivery into cells. Part III discusses current available methods, including adjuvant and prime-boost approaches, for enhancing the potency of DNA vaccines. Part IV describes several key areas of application in the field, including allergy, avoidance of autoimmunity, and neonate and infant vaccine response. DNA Vaccines: Methods and Protocols, Second Edition concludes with a review of protocols for vaccine production and purification, and applicable quality control methods. This collection of up-to-date protocols demonstrates the tremendous progress that has been made in the field of DNA vaccination since the initial appearance of this new vaccination strategy. Given current enthusiasm for the magnificent potential for this technology among the scientific community, researchers will find tremendous value in this updated and enhanced molecular methods guide.
Title:DNA VaccinesFormat:PaperbackDimensions:400 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617375969

ISBN - 13:9781617375965

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Table of Contents

Part 1: DNA Vaccine DesignDNA Vaccine DesignJanet BrandsmaDesign of Plasmid DNA Constructs for VaccinesDonna L. Montgomery and Kristala Jones-PratherVaccination with Messenger RNASteve PascoloA Stress Protein-Facilitated Antigen Expression System for Plasmid DNA VaccinesPetra Riedl, Nicolas Fissolo, Joerg Reimann, and Reinhold SchirmbeckIn Vitro Assay of Immunostimulatory Activities of Plasmid VectorsWeiwen Jiang, Charles Rich, and David PisetskyPart 2: DNA Vaccine Delivery SystemsDelivery of DNA Vaccines Using ElectroporationShawn Babiuk, Sylvia van Drunen Little-van den Hurk, and Lorne BabiukNeedle-Free Injection of DNA VaccinesKanakatte Raviprakash and Kevin PorterNeedle-Free Delivery of Veterinary DNA VaccinesSylvia van Drunen Little-van den Hurk, Shawn Babiuk, and Lorne BabiukSurface-Modified Biodegradable Microspheres for DNA Vaccine DeliveryMark Keegan and Mark SaltzmanA Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Non-Viral Hybrid ApproachDan Luo, Yougen Li, Soong Ho Um, and Yen CuIdentification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect ImmunofluorescenceAnne Doody and David PutnamPart 3: DNA Vaccine Adjuvants and Activity EnhancementAdjuvant Properties of CpG Oligonucleotides in PrimatesDaniela VerthelyiComplexes of DNA Vaccines with Cationic, Antigenic Peptides are Potent, Polyvalent CD8+ T Cell-Stimulating AgentsPetra Riedl, Joerg Reimann, and Reinhold SchirmbeckPrime Boost Strategies in DNA VaccinesC. Jane Dale, Scott Thomson, Robert De Rose, Charani Ranasinghe, C. Jill Medveczky, Joko Pamungkas, David B. Boyle, Ian A. Ramshaw, and Stephen J. KentModifying Professional Antigen Presenting Cells to Enhance DNA Vaccine PotencyC.-F. Hung, M. Yang, and T.C. WuReplicase-Based DNA Vaccines for Allergy TreatmentSandra Scheiblhofer, Richard Weiss, Maximilian Gabler, Wolfgang Leitner, and Josef ThalhamerPart 4: DNA Vaccine ApplicationsImmunological Responses of Neonates and Infants to DNA VaccinesMartha Sedegah and Stephen L. HoffmanDNA Vaccines for Allergy TreatmentRichard Weiss, Sandra Scheiblhofer, and Josef ThalhamerProtection From Autoimmunity by DNA Vaccination Against T Cell Receptor (TCR)Thorsten Buch and Ari WaismanUse of Bone Marrow-Chimeric Mice in Elucidating Immune MechanismsAkiko IwasakiPart 5: DNA Vaccine Production, Purification, and QualityA Simple Method for the Production of Plasmid DNA in BioreactorsK. Listner, L. Kiser Bentley, and Michael ChartrainPractical Methods for Supercoiled pDNA ProductionJohn BallantyneProduction of Plasmid DNA in Industrial Quantities According to cGMP GuidelinesJoachim Schorr, Peter Moritz, Astrid Breul, and Martin ScheefLarge-Scale Non-Chromatographic Purification of Plasmid DNAJason Murphy and Sangeetha SagarAssuring the Quality, Safety and Efficacy of DNA VaccinesJames S. Robertson

Editorial Reviews

From the reviews:"...does an excellent job of describing the current methodologies in their development, what clinical benefit can be expected, and what is known of their mode of action...has thoroughly covered the current state of the art." -Antiviral Research"The present book contains 41 chapters by experts in the field not only describing the state-of-the-art thinking on each topic, but, more importantly, providing a step-by-step recipe for constructing the cost effective plasmids and measuring host immune responses, both humoral and cellular, as well as all the stops in between including delivery, etc....an excellent roadmap as to how to proceed...This comprehensive collection of chapters is the first of its kind in the rapidly moving field to provide an excellent update of all of the recent advances and, more importantly, the actual technical approaches to developing vaccines. This is a book that should be in everyone's library who is interested in pursing studies employing DNA immunization and for graduate students interested in an up-to-date understanding of DNA vaccination." -FEBS Letters"Its 41 chapters provide a valuable introduction, a series of working protocols, and a set of heavily referenced reviews of state-of-the-art methods in the production, use, and analysis of DNA vaccines. The various chapters are especially fruitful in their presentation of detailed techniques for making buffers and complex biological reagents." -Modern Drug Discovery"Tecular Biology series, consists of 41 well written and referenced chapters dealing with certain aspects of DNA vaccination. The subjects addressed range from large scale plasmid isolation to DNA vaccination and autoimmunity, and illuminate the explosive growth this field has undergone in recent years. A substantial amount of the text concentrates on the practical aspects of processing DNA, quality control and immunological responses....In short, this book is a valuable addition to the library of all people actively involved in the field of DNA vaccination. Researchers will find the detailed protocols and innumerable reference valuable. I would recommend this book to any researcher in this field, if only to finally have a book with well organized and referenced protocols instead of a pile of coffee stained photocopies." -Today's Life Science"The scope of the text is wide and covers virtually all aspects of the subject from the initial plasmid preparation and industrial scale up to the regulatory issues that will probably need to be addressed if such vaccines reach licensing stage." - The Journal of Medical Microbiology"Work on the development of DNA vaccines continues aspace for a huge range of potential applications and this book will be invaluable for any laboratory involved in research in this field." -Microbiology Today"It is packed with technical and scientific information that will be helpful to scientists working on vaccines. . It is written for researchers in the field of vaccine development and those delving into the production of these vaccines. . it is written clearly and is easy to follow . . The research presented is exciting and is offered with clear explanations and rationale. . This well organized book takes the reader from the conception of the vaccine to the production . ." (Rebecca Horvat, Doody's Book Review Service, January, 2007)