Drosophila Melanogaster: Practical Uses In Cell And Molecular Biology

Other | January 1, 1995

byWilson, Leslie, Leslie Wilson

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Drosophila melanogaster: Practical Uses in Cell and Molecular Biologyis a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. It also contains a wealth of technical information for beginning or advanced Drosophila workers. Chapters, written within a year of publication, make this topical volume a valuable laboratory guide today and an excellent general reference for the future.

Key Features
* Collection of ready-to-use, state-of-the art methods for modern cell biological and related research usingDrosophila melanogaster
* Accessible to both experienced Drosophila researchers and to others who wish to join in at the cutting edge of this system
* Drosophila offers an easily managed life cycle, inexpensive lifestyle, extraordinarily manipulable molecular and classical genetics, now combined with powerful new cell biology techniques
* Introduction and overview sections orient the user to the Drosophila literature and lore
* Six full-color plates and over 100 figures and tables enhance the understanding of these cell biology techniques

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From the Publisher

Drosophila melanogaster: Practical Uses in Cell and Molecular Biologyis a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomencl...

Format:OtherDimensions:755 pages, 1 × 1 × 1 inPublished:January 1, 1995Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0080859429

ISBN - 13:9780080859422

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Extra Content

Table of Contents

Preface: Why the Fly?
Basic Genetic Methodologies:
L.S.B. Goldstein,Sources of Information about the Fly: Where to Look It Up.
K.A. Matthews,Care and Feeding ofDrosophila melanogaster.
M.F. Wolfner and M.L. Goldberg,Harnessing the Power ofDrosophilaGenetics.
B.A. Hamilton and K. Zinn,From Clone to Mutant Gene.
Isolation and Culture Methods:
C.D. Shaffer, J.M. Wuller, and S.C.R. Elgin,Raising Large Quantities ofDrosophilafor Biochemical Experiments.
J.E. Natzle and G.D. Vesenka,Isolation and Organ Culture of Imaginal Tissues.
A.P. Mahowald,Mass Isolation of Fly Tissues.
S. Cumberledge and M. Krasnow,Preparation and Analysis of Pure Cell Populations fromDrosophila.
L. Cherbas, R. Moss, and P. Cherbas,Transformation Techniques forDrosophilaCell Lines.
Biochemical Preparation Methods:
C.D. Shaffer, J.M. Wuller, and S.C.R. Elgin,Preparation ofDrosophilaNuclei.
M.J. Matunis, E.L. Matunis, and G. Dreyfuss,Isolation and Characterization of RNA-Binding Proteins fromDrosophila melanogaster.
P.B. Becker, T. Tsukiyama, and C. Wu, Chromatin Assembly Extracts fromDrosophiaEmbryos.
R.T. Kamakaka and J.T. Kadonaga, The Soluble Nuclear Fraction, a Highly Efficient Transcription Extract fromDrosophilaEmbryos.
E.A. Fyrberg, S.I. Bernstein, and K.V. Raghavan, Basic Methods forDrosophilaMuscle Biology.
K.G. Miller and D.R. Kellogg, Isolation of Cytoskeletal Proteins fromDrosophila.
W.M. Saxton, Isolation and Analysis of Microtubule Motor Proteins.
M. Hortsch, Preparation and Analysis of Membranes and Membrane Proteins fromDrosophila.
J.H. Fessler, R.E. Nelson, and L.I. Fessler, Preparation of Extracellular Matrix.
Cytological Methods:
M.-L. Pardue, Looking at Polytene Chromosomes.
D.J. Andrew and M.P. Scott, Immunological Methods for Mapping Protein Distributions on Polytene Chromosomes.
M. Gatti, S. Bonaccorsi, and S. Pimpinelli, Looking atDrosophilaMitotic Chromosomes.
S. Gunawardena and M. Rykowski, Looking at Diploid Interphase Chromosomes.
K.L. McDonald, Electron Microscopy and EM Immunocytochemistry.
N.H. Patel, Imaging Neuronal Subsets and Other Cell Types in Whole-MountDrosophilaEmbryos and Larvae Using Antibody Probes.
W.E. Theurkauf, Immunofluorescence Analysis of the Cytoskeleton during Oogenesis and Early Embryogenesis.
D.P. Kiehart, R.A. Montague, W.L. Rickoll, G.H. Thomas, and D. Foard, High-Resolution Microscopic Methods for the Analysis of Cellular Movements inDrosophilaEmbryos.
C.H. Girdham and P.H. O'Farrell, The Use of Photoactivatable Reagents for the Study of Cell Lineage inDrosophilaEmbryogenesis.
E. Verheyen and L. Cooley, Looking at Oogenesis.
Analysis of Gene Expressionin Situ:
A.J. Andres and C.S. Thummel, Methods for Quantitative Analysis of Transcription in Larvae and Prepupae.
R. Lehmann and D. Tautz,In SituHybridization to RNA.
Z. Zachar, J. Kramer, and P.M. Bingham, Looking at mRNA Splicing and Transportin Situ.
A. Beyer, M. Sikes, and Y. Osheim, EM Methods for Visualization of Genetic Activity from Disrupted Nuclei.
Molecular and Classical Genetic Analysis:
A.H. Brand, A.S. Manoukian, and N. Perrimon, Ectopic Expression inDrosophila.
T. Xu and S.D. Harrison, Mosaic Analysis Using FLP Recombinase.
A.J. Bieber, Analysis of Cellular Adhesion in Cultured Cells.
G. Schubiger and B. Edgar, Using Inhibitors to Study Embryogenesis.
A.E.L. Beermann and D.G. Jay, Chromophore-Assisted Inactivation of Cellular Proteins.
Chapter References.
Index.
Volumes in Series.