Drugs of Abuse: Neurological Reviews and Protocols by John Q. WangDrugs of Abuse: Neurological Reviews and Protocols by John Q. Wang

Drugs of Abuse: Neurological Reviews and Protocols

EditorJohn Q. Wang

Paperback | November 9, 2010

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A cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail by hands-on experts, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Insightful review articles address key current topics on the effect of drugs of abuse on the brain.
Title:Drugs of Abuse: Neurological Reviews and ProtocolsFormat:PaperbackDimensions:528 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373281

ISBN - 13:9781617373282

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Table of Contents

Part I. Special ReviewsThe Temporal Sequence of Changes in Gene Expression by Drugs of AbusePeter W. Kalivas, Shigenobu Toda, M. Scott Bowers, David A. Baker, and M. Behnam GhasemzadehEffects of Psychomotor Stimulants on Glutamate Receptor ExpressionMarina E. WolfAdult Neural Stem/Progenitor Cells in the Forebrain: Implications for Psychostimulant Dependence and MedicationJohn Q. Wang, Limin Mao, and Yuen-Sum LauNeuroprotective Effect of Naloxone in Inflammation-Mediated Dopaminergic Neurodegeneration: Dissociation from the Involvement of Opioid ReceptorsBin Liu and Jau-Shyong HongNeuropharmacology and Neurotoxicity of 3,4-MethylenedioxymethamphetamineGary A. Gudelsky and Bryan K. YamamotoLearning and Memory Mechanisms Involved in Compulsive Drug Use and RelapseJoshua D. BerkeFrom Drugs of Abuse to Parkinsonism: The MPTP Mouse Model of Parkinson's DiseaseYuen-Sum Lau and Gloria E. MeredithPart II. Detection of mRNA Expression in the StriatumIn Situ Hybridization with Isotopic Riboprobes for Detection of Striatal Neuropeptide mRNA Expression After Dopamine Stimulant AdministrationYasmin L. HurdCombining In Situ Hybridization with Retrograde Tracing and Immunohistochemistry for Phenotypic Characterization of Individual NeuronsCatherine Le MoineAnalysis of mRNA Expression Using Double In Situ Hybridization Labeling with Isotopic and Nonisotopic ProbesJohn Q. WangQuantification of mRNA in Neuronal Tissue by Northern AnalysisChristine L. KonradiAnalysis of Gene Expression in Striatal Tissue by Multiprobe RNase Protection AssayNeil M. RichtandAnalysis of mRNA Expression in Striatal Tissue by Differential Display Polymerase Chain ReactionJoshua D. BerkeSemiquantitative Real-Time PCR for Analysis of mRNA LevelsStephen J. Walker, Travis J. Worst, and Kent E. VranaApplication of TaqMan RT-PCR for Real-Time Semiquantitative Analysis of Gene Expression in the StriatumAndrew D. Medhurst and Menelas N. PangalosSystematic Screening of Gene Expression Using a cDNA MacroarrayTravis J. Worst, Willard M. Freeman, Stephen J. Walker, and Kent E. VranaPart III. Detection of Protein Expression in the StriatumQuantification of Protein in Brain Tissue by Western Immunoblot AnalysisChristine L. KonradiImmunohistochemical and Immunocytochemical Detection of Phosphoproteins in Striatal NeuronsLimin Mao and John Q. WangAnalysis of Protein Expression in Brain Tissue by ELISASteffany A. L. Bennett and David C. S. RobertsDopamine Receptor Binding and Quantitative Autoradiographic StudyBeth LevantAnalysis of DNA-Binding Activity in Neuronal Tissue with the Electrophoretic Mobility-Shift AssayChristine L. KonradiPart IV. Gene Function AnalysisViral-Mediated Gene Transfer to Study the Behavioral Correlates of CREB Function in the Nucleus Accumbens of RatsWilliam A. Carlezon, Jr. and Rachael L. NeveGenerating Gene Knockout Mice for Studying Mechanisms Underlying Drug AddictionJianhua Zhang and Ming XuAntisense Approaches in Analyzing the Functional Role of Proteins in the Central Nervous SystemJohn Q. Wang and Limin MaoPart V. Striatal Culture PreparationPrimary Striatal Neuronal CultureLimin Mao and John Q. WangPrimary Rat Mesencephalic Neuron-Glia, Neuron-Enriched, Microglia-Enriched, and Astroglia-Enriched CulturesBin Liu and Jau-Shyong HongPrimary Culture of Adult Neural ProgenitorsSteffany A. L. Bennett and Lysanne Melanson-DrapeauOrganotypic Culture of Developing Striatum: Pharmacological Induction of Gene ExpressionFu-Chin LiuPart VI. Detection of Transmitter Release in the StriatumMicrodialysis Coupled with Electrochemical Detection: A Way to Investigate Brain Monoamine Role in Freely Moving AnimalsEzio CarboniHPLC/EC Detection and Quantification of Acetylcholine in DialysatesJames A. Zackheim and Elizabeth D. AbercrombieReal-Time Measurements of Phasic Changes in Extracellular Dopamine Concentration in Freely Moving Rats by Fast-Scan Cyclic VoltammetryPaul E. M. Phillips, Donita L. Robinson, Garret D. Stuber, Regina M. Carelli, and R. Mark WightmanMeasurement of Dopamine Uptake in Neuronal Cells and TissuesYuen-Sum LauPart VII. Behavioral Assessment and OthersLaboratory Analysis of Behavioral Effects of Drugs of Abuse in RodentsNeil M. RichtandConditioned Place Preference: A Simple Method for Investigating Reinforcing Properties in Laboratory AnimalsEzio Carboni and Cinzia VaccaDetection of Cell Proliferation and Cell Fate in Adult CNS Using BrdU Double-Label ImmunohistochemistryZin Z. Khaing and Mariann BlumIndex

Editorial Reviews

"It describes in detail laboratory methods used by researchers working in the area of drug abuse and drug addiction. This is a unique book. Few books deal in detail with the methods underlying experiments. It will be of primary value to active investigators with active laboratories."-Doody's Health Sciences Book Review Journal