Enzyme Assays

Paperback | February 1, 2002

EditorRobert Eisenthal, Michael J. Danson

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Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gelelectrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening;statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

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Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reactio...

Robert Eisenthal is in the Department of Biology and Biochemistry, University of Bath, UK. Michael Danson is in the Department of Biology and Biochemistry, University of Bath, UK.

other books by Robert Eisenthal

Format:PaperbackDimensions:302 pages, 9.69 × 7.44 × 0.69 inPublished:February 1, 2002Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199638209

ISBN - 13:9780199638208

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Table of Contents

1. Keith F Tipton: Principles of enzyme assay and kinetic studies2. Robert A John: Photometric assays3. Kelvin T Hughes: Radiometric assays4. Shabih E H Syed: High performance liquid chromatography assays5. J B Clark: Electrochemical assays: the oxygen electrode6. R D Hurst and J B Clark: Electrochemical assays: the nitric oxide electrode7. Keith Brocklehurst: Electrochemical assays: the pH-stat8. Gunter M Rothe: Enzyme assays after gel electrophoresis9. Nicholas C Price and Lewis Stevens: Techniques for enzyme extraction10. Mark T Martin: Determination of active site concentration11. David Hayes and Geoff Mellor: High throughput screening - considerations for enzyme assays12. Athel Cornish-Bowden: Statistical analysis of enzyme kinetic data