Epigenetics Protocols by Trygve TollefsbolEpigenetics Protocols by Trygve Tollefsbol

Epigenetics Protocols

EditorTrygve Tollefsbol

Paperback | November 19, 2010

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A collection of state-of-the-art methods for epigenetic analysis, including recent breakthrough techniques that have great potential in the rapidly expanding field of non-Mendelian genetics. The authors provide techniques for the analysis of chromatin remodeling, such as histone acetylation and methylation. In addition, methods in newly developed and especially promising areas of epigenetics, such as telomere position effects, quantitative epigenetics, and ADP ribosylation are covered. There is also an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure. Of special interest are potentially revolutionary techniques. These include methods for determining changes in native chromatin, methods of microarray analysis applied to epigenetics, and methylation-sensitive single-strand conformation techniques. The methods are suitable not only for studying fundamental biological processes, but also for investigating possible therapeutic interventions and such diseases as cancer.
Title:Epigenetics ProtocolsFormat:PaperbackDimensions:320 pages, 9.02 × 5.98 × 0.68 inPublished:November 19, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617375012

ISBN - 13:9781617375019

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Table of Contents

Methods of Epigenetic AnalysisTrygve O. TollefsbolChromatin Immunoprecipitation AssaysYan Yan, Haobin Chen, and Max CostaNative Chromatin ImmunoprecipitationAlan W. Thorne, Fiona A. Myers, and Tim R. HebbesQ-PCR in Combination With ChIP Assays to Detect Changes in Chromatin AcetylationRyan A. Irvine and Chih-Lin HsiehRestriction Endonuclease Accessibility as a Determinant of Altered Chromatin StructureWilliam M. Hempel and Pierre FerrierMeasuring Changes in Chromatin Using Micrococcal NucleaseNicolas Steward and Hiroshi SanoDNaseI Hypersensitivity Analysis of Chromatin StructureQianjin Lu and Bruce RichardsonInhibition of Histone DeacetylasesCheng Liu and Dawei XuSite-Specific Analysis of Histone Methylation and AcetylationDavid Umlauf, Yuji Goto, and Robert FeilAnalysis of Mammalian Telomere Position EffectJoseph A. Baur, Woodring E. Wright, and Jerry W. ShayActivity Assays for Poly-ADP Ribose Polymerase 1Eva Kirsten, Ernest Kun, Jerome Mendeleyev, and Charles P. OrdahlMultigenerational Selection and Detection of Altered Histone Acetylation and Methylation Patterns: Toward a Quantitative Epigenetics in DrosophilaMark D. Garfinkel, Vincent E. Sollars, Xiangyi Lu, and Douglas M. RudenProfiling DNA Methylation by Bisulfite Genomic Sequencing: Problems and SolutionsLiang Liu, Rebecca C. Wylie, Nathaniel J. Hansen, Lucy G. Andrews, and Trygve O. TollefsbolMethylation-Sensitive Single-Strand Conformation Analysis: A Rapid Method to Screen for and Analyze DNA MethylationJean Benhattar and Geneviève ClémentSIRPH Analysis: SNuPE With IP-RP-HPLC for Quantitative Measurements of DNA Methylation at Specific CpG SitesOsman El-MaarriReal-Time PCR-Based Assay for Quantitative Determination of Methylation StatusUlrich Lehmann and Hans KreipeDenaturing Gradient Gel Electrophoresis to Detect Methylation Changes in DNAMasahiko ShiraishiPhotocrosslinking Oligonucleotide Hybridization Assay for Concurrent Gene Dosage and CpG Methylation AnalysisRisa Peoples, Michael Wood, and Reuel Van AttaMethylation-Specific Oligonucleotide MicroarrayPearlly S. Yan, Susan H. Wei, and Tim Hui-Ming HuangMethylation-Specific PCR In Situ HybridizationGerard J. NuovoRelative Quantitation of DNA Methyltransferase mRNA by Real-Time RT-PCR AssayJohn Attwood and Bruce RichardsonDMB (DNMT-Magnetic Beads) Assay: Measuring DNA Methyltransferase Activity In VitroTomoki Yokochi and Keith D. RobertsonIndex

Editorial Reviews

"Comprehensive and easy to use...offers investigators readily reproducible techniques that will further promote progress in this critically important field." - Tumori