Epithelial Cell Culture: A Practical Approach

Paperback | April 30, 1999

EditorAndrew J. Shaw

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This book describes methods of culturing epithelial cells so as to mimic accurately their behaviour in vivo, and the resulting model systems can be used in a wide range of research areas: pharmaceutics, pharmacology, toxocology, and cell biology. The culture technique involves growing cells ona porous substrate, so that the cells can receive nutrients by diffusion from the underlying layer, as they would in vivo.

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This book describes methods of culturing epithelial cells so as to mimic accurately their behaviour in vivo, and the resulting model systems can be used in a wide range of research areas: pharmaceutics, pharmacology, toxocology, and cell biology. The culture technique involves growing cells ona porous substrate, so that the cells can r...

From the Jacket

Our understanding of the functions of epithelia, and their development and maintenance, has been rapidly advanced by the use of cell cultures which faithfully display the morphological, biochemical, and functional characteristics of these tissues. This book gives detailed step-by-step descriptions of the methods used to mimic epithelia...

Andrew J. Shaw is at University of London.

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Format:PaperbackDimensions:238 pages, 9.21 × 6.14 × 0.55 inPublished:April 30, 1999Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635722

ISBN - 13:9780199635726

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Table of Contents

1. Modelling epithelial tissues2. Identification and localization of cytokeratins3. Assessment of cell polarity4. Xenobiotic metabolism in epithelial cells5. Culture and characterization of human endothelial cells6. Studying transport processes in aborptive epithelia7. A model of glandular epithelium for studying secretion8. A model of the blood-testes barrier for studying testicular toxicity9. Reconstruction of human skin epidermis in vitroAppendix 1. Addresses of suppliersAppendix 2. An in vitro model of the blood-brain barrier for studying drug transport to the brain

Editorial Reviews

`this handy volume comes at an opportune time ... It will be valuable to new PhD students working in this area, and also to project students.'M. Taylor, Biochemical Education 25(1) 1997