Epithelial Cell Culture Protocols by Clare WiseEpithelial Cell Culture Protocols by Clare Wise

Epithelial Cell Culture Protocols

EditorClare Wise

Paperback | November 10, 2010

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Well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Epithelial Cell Culture Protocols offers a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.
Title:Epithelial Cell Culture ProtocolsFormat:PaperbackDimensions:424 pages, 9.02 × 5.98 × 0.01 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372412

ISBN - 13:9781617372414

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Table of Contents

Human Lens Epithelial Cell CultureNobuhiro IbarakiHuman Airway Epithelial Cell CultureMutsuo Yamaya, Masayoshi Hosoda, Tomoko Suzuki, Norihiro Yamada, and Hidetada SasakiRat Gastric Mucosal Epithelial Cell CultureYoshitaka Konda and Tsutomu ChibaThymic Epithelial Cell CultureCarsten RöpkeBile Duct Epithelial Cell CultureAlphonse E. SiricaLiver Epithelial Cell CultureRolf GebhardtPulmonary Epithelial Cell CultureBen ForbesProstate Epithelial Cell Isolation and CultureDavid L. HudsonA Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk BarrierAlbert Guidry and Celia O'BrienThe Blood-CSF Barrier in Culture: Development of a Primary Culture and Transepithelial Transport Model for Choroidal Epithelial CellsWei Zheng and Qiuqu ZhaoAn In Vitro Model of Differentiated Human Airway Epithelia: Methods for Establishing Primary CulturesPhilip H. Karp, Thomas O. Moninger, S. Pary Weber, Tamara S. Nesselhauf, Janice L. Launspach, Joseph Zabner, and Michael J. WelshPrimary Mouse Keratinocyte CultureKairbaan Hodivala-DilkeAnalyzing Apoptosis in Cultured Epithelial CellsAndrew P. Gilmore and Charles H. StreuliKeratins as Markers of Epithelial CellsDavid L. HudsonEpithelial Cell IntegrinsJoachim RychlyIsolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free MediumSebastian Zellmer and Dieter ReissigClinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin SurfacesNorio KumagaiTransplantation of Cultured Epithelial CellsMinoru Ueda, Yukio Sumi, Yoshitaka Hibino, and Ken-Ichiro HataAn Outgrowth Culture System of Normal Human Epithelium: An In Vitro Model to Study the Coordination of Cellular Migration and ProliferationTohru MasuiApplication of Epithelial Cell Culture in Drug Transport in the Respiratory TractJie Shen and Vincent H. L. LeeApplications of Epithelial Cell Culture in Studies of Drug TransportStaffan Tavelin, Johan Gråsjö, Jan Taipalensuu, Göran Ocklind, and Per ArturssonX-Ray Microanalysis of Epithelial Cells in CultureGodfried M. RoomansENU Mutagenesis of Rat Mammary Epithelial CellsGeorge StoicaENU-Induced Ovarian CancerGeorge StoicaMeasurement of Membrane Capacitance in Epithelial MonolayersCarol A. Bertrand and Ulrich HopferAssessing Epithelial Cell Confluence by SpectroscopySimon A. LewisCo-Cultivation of Liver Epithelial Cells with HepatocytesRolf GebhardtCo-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular CalciumWee Soo Shin, Chieko Hemmi, and Teruhiko Toyo-okaEstablishing Epithelial-Immune Cell Co-Cultures: Effects on Epithelial Ion Transport and PermeabilityDerek M. McKay and Mary H. PerdueExplant Cultures of Embryonic Epithelium: Analysis of Mesenchymal SignalsCarin Sahlberg, Tuija Mustonen, and Irma ThesleffBacterial Interactions with Host Epithelium In VitroNicola Jones, Mary H. Perdue, Philip M. Sherman, and Derek M. McKay

Editorial Reviews

"The book could be used effectively by experienced or novice basic science researchers or clinicians since the protocols are written clearly and concisely. There are 31 brief chapters in this book and about 60 authors. They are experienced and knowledgeable researchers in the field. . .The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to culture these cells. . .would be quite useful for anyone wishing to place epithelial cells in culture."-Doody's Health Sciences Book Review Journal