Essential Developmental Biology: A Practical Approach: A Practical Approach by Claudio D. Stern

Essential Developmental Biology: A Practical Approach: A Practical Approach

EditorClaudio D. Stern, Peter W. H. Holland

Paperback | July 1, 1995

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Developmental biology is a newly-developing subject that is attracting much interest because of exciting findings being made using a combination of classical embryo manipulation with more modern techniques. This book brings together for the first time, and in comprehensive, easy-to-followprotocols, practical instructions for all of the main techniques, from traditional embryology to cellular and molecular methods. It includes complete reprints of all the stage tables in common use for the main laboratory species. It will become an essential addition to laboratory benches of thoseusing any technique as applied to embryos, cells, or tissues. It will also help teachers to design practical classes suitable for undergraduate and graduate courses.

About The Author

Claudio D. Stern, University Lecturer, Department of Human Anatomy, University of Oxford. Peter W. H. Holland, Royal Society University Research Fellow, Department of Zoology, University of Oxford.
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Title:Essential Developmental Biology: A Practical Approach: A Practical ApproachFormat:PaperbackDimensions:362 pages, 9.21 × 6.14 × 0.03 inPublished:July 1, 1995Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:019963422X

ISBN - 13:9780199634224

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Table of Contents

Part I: Basic techniques: obtaining and handling embryos1. Drosophila embryos2. Nematode embryos3. Embryos and larvae of invertebrate deuterostomes4. Zebrafish embryos5. Xenopus6. Avian embryos7. Post-implantation mammalian embryos8. Simple tips for photomicrography or embryosPart II: Experimental Embryology9. Fate maps and cell lineage analysis10. Microinjection into Drosophila embryos11. Cell transplantation in Xenopus12. Transplantation in avian embryos13. Chick limb buds14. Nuclear transplantation15. Laser ablation of cellsPart III: Cellular techniques16. Studying cell movements in vivo by time-lapse video microscopy17. Induction assays in Xenopus18. Culture of avian neural crest cells19. ES cells and gene targeting20. Introduction of genes using retroviral vectorsPart IV: Molecular techniques21. Immunocytochemistry of embryonic material22. RNase protection assays23. cDNA library construction24. Analysis of gene expression by RT-PCR25. Cloning genes using PCR26. In situ hybridisationAppendicesI: Stage tablesa. Westerfield for zebrafish embryosb. Nieukoop and Faber for Xenopus laevisc. Eyal-Giladi and Kochav for early chickd. Hamburger and Hamilton for later chickII: Suppliers of specialist itemsIndex

Editorial Reviews

'this book will be widely welcomed ... the ability to tackle a developmental problem in a variety of different species is increasingly valuable; having the relevant methods to hand will make this type of approach easier and more productive ... highly recommended and sensibly priced for thebiologist on a shoestring.'Justin Grindley, TIG, September 1994, Vol. 10. No. 9