Essential Molecular Biology Vol 2

Paperback | November 15, 2001

EditorTerry Brown

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The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combinesolid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements.Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the samedown to earth fashion with protocols complemented by background information and troubleshooting hints.

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The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combinesolid practical information with sufficient ...

Terry Brown is at UMIST, Manchester, UK.

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Format:PaperbackDimensions:310 pages, 9.69 × 7.44 × 0.71 inPublished:November 15, 2001Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199636443

ISBN - 13:9780199636440

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Table of Contents

1. T.A. Brown: Strategies for research in molecular biology2. Anna-Maria Frischauf: Construction of genomic libraries in lambda and cosmic vectors3. Madan K. Bhattacharyya: Construction of a cDNA library4. C.R.Mundy, M. W.Cunningham and C.A.Read: Nucleic acid labelling and detection5. N.J.Dyson: Immobilization of nucleic acids and hybridization analysis6. T.A.Brown: DNA sequencing7. T.A.Brown: The polymerase chain reaction8. John M. Ward: Protein expression in Escherichia coli9. Colin P.Smith: Transcript mappingAppendices1. Health hazards and safety procedures2. Equipment for molecular biology research3. Important Escherichia coli strains4. Recipes and general procedures5. Restriction endonucleases6. DNA and RNA modification enzymes7. List of suppliers' addresses