Exon Skipping: Methods and Protocols by Annemieke Aartsma-RusExon Skipping: Methods and Protocols by Annemieke Aartsma-Rus

Exon Skipping: Methods and Protocols

EditorAnnemieke Aartsma-Rus

Hardcover | March 28, 2012

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"Next generation" sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate "real polymorphisms" from mutations that affect splicing. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping.
Title:Exon Skipping: Methods and ProtocolsFormat:HardcoverDimensions:451 pagesPublished:March 28, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617797669

ISBN - 13:9781617797668

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Table of Contents

Part I: Mutations1.   DNA Diagnostics and Exon SkippingUmasuthan Srirangalingam and Shern L. Chew2. Bioinformatics and Mutations Leading to Exon SkippingF.O. Desmet and C. Béroud3. Minigenes to Confirm Exon Skipping MutationsLourdes R. Desviat, Belén Pérez & Magdalena Ugarte4.Analysis and Interpretation of RNA Splicing Alterations in Genes Involved in Genetic Disorders Maaike P.G. Vreeswijk and Heleen M. van der Klift5. Exon Skipping Mutations in NeurofibromatosisEmanuele Buratti and Diana BarallePart II: Tools to Induce Exon Skipping6. Overview on Applications of Antisense-mediated Exon SkippingWilleke van Roon-Mom and Annemieke Aartsma-Rus 7. Overview on DMD Exon SkippingAnnemieke Aartsma-Rus8. Overview on AON DesignAnnemieke Aartsma-Rus9. Optimizing RNA/ENA Chimeric Antisense Oligonucleotides Using in vitro SplicingYasuhiro Takeshima, Mariko Yagi, Masafumi Matsuo10. Optimizing Antisense Oligonucleotides using Phosphorodiamidate Morpholino OligomersLinda J Popplewell, Alberto Malerba, and George Dickson11. Optimizing Splice Switching Oligomer Sequences using 2'-O-methyl Phosphorothioate ChemistryCarl Adkin, Sue Fletcher and Steve D Wilton12. Exon Skipping Quantification by Real Time PCRAlessandra Ferlini and Paola Rimessi13. Antisense-mediated Exon Skipping to Shift Alternative Splicing to Treat CancerJing Wan14. Antisense-mediated Exon Skipping to Generate Soluble ReceptorsA. Seda Yilmaz-Elis and J. Sjef Verbeek15. Antisense-mediated Exon Skipping to Reframe TranscriptsSandrina Turczynski, Matthias Titeux, Nathalie Pironon, and Alain Hovnanian16. U1 snRNA as an Effective Vector for Stable Expression of Antisense Molecules andfor the Inhibition of the Splicing ReactionMartone Julie, De Angelis Fernanda Gabriella and Bozzoni Irene17. Engineering U7snRNA Gene to Reframe Transcripts Aurélie Goyenvalle 18. Dynamic Fluorescent and Luminescent Reporters for Cell Based Splicing ScreensClaude C. Warzecha, Ruben Hovhannisyan, and Russ P. Carstens19. Antisense-mediated Exon Skipping to Induce Gene KnockdownPetra Disterer and Bernard Khoo20. Antisense-mediated Exon InclusionYimin Hua and Adrian R. Krainer21. Antisense Genes to Induce Exon InclusionR. Nlend Nlend & D. Schümperli22. Using Mini-genes to Identify Factors that Modulate Alternative Splicing Robert Morse, Adrian G. Todd and Philip J. YoungPart III: Delivery23. Overview of Alternative Oligonucleotide Chemistries for Exon SkippingAmer F. Saleh, Andrey A. Arzumanov and Michael J. Gait24. Identification of Peptides for Tissue Specific DeliveryHans Heemskerk25. Systemic Delivery of Antisense Oligomer in Animal Models and its Implications for Treating DMDQi Long Lu and Bo Wu26. Cell-penetrating Peptides Enhance Systemic Delivery of Antisense Morpholino Oligomers Hong M Moulton 27. Optimizing Tissue-specific Antisense Oligonucleotide-peptide ConjugatesCorinne A Betts, Suzan M Hammond, Hai-fang Yin, and Matthew JA Wood