Experimental Protocols for Reactive Oxygen and Nitrogen Species

Hardcover | June 1, 2000

EditorNaoyuki Taniguchi, John Gutteridge

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Experimental Protocols for Reactive Oxygen and Nitrogen Species is a translated, expanded, and fully updated of the Japanese book Experimental Protocols for Reactive Oxygen Research: assay methods, gene analysis, and pathophysiology models published in 1994. The aim of the book is to provideexperimental protocols covering many aspects of free radical research: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. The protocols are all self-contained describing the equipment and reagents needed and then detailing the experimental procedure.

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From the Publisher

Experimental Protocols for Reactive Oxygen and Nitrogen Species is a translated, expanded, and fully updated of the Japanese book Experimental Protocols for Reactive Oxygen Research: assay methods, gene analysis, and pathophysiology models published in 1994. The aim of the book is to provideexperimental protocols covering many aspects ...

Naoyuki Taniguchi is at Osaka University Medical School. John Gutteridge is at Royal Brompton and Harefield NHS Trust, London.

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Format:HardcoverPublished:June 1, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0198506686

ISBN - 13:9780198506683

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Table of Contents

Part One: MeasurementROS and RNS measurementsMeasurements for proteinaceous plasma antioxidants, low Mw antioxidants, and REDOX related compoundsMeasurements for specific ROS and RNS enzymes and related proteinsLipids and lipid peroxidizationIron and reactive iron speciesCopper and reactive copper speciesPart Two: Gene AnalysesResearch for gene expressions of ROS and RNS related genesDNA damage and repairPart Three: Experiments for pathophysiological modelsIsolated and perfused hearts (Langendorf's model)Myocardial ischaemia-reperfusion models in vivoGI tracts in vivoBrain ischaemiaCell cultureAthersclerosisDicarbonyls and HB-EGF gene expression UV damageAgeingOvulationScavenging NO in experimental animalsUsing radioimmuno assays in brain slicesOxidative stressCarcinogenesisIron overloadCopper overload

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`An "enjoyable and informative book [...] Lane presents a nicely crafted account of an important element's place in our lives. His book deserves to be widely read..."'Nature