FISH (Fluorescence In Situ Hybridization)

Paperback | May 1, 2002

EditorBarbara G Beatty, Sabine Mai, Jeremy A Squire

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This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols ina systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probeproduction and labelling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics. The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensiveand up to date bibliography providing background to all the advanced molecular cytogenetics The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecularcytogenetics techniques in current use. This book will be of particular value to scientists, technologists, and students in the areas of molecular pathology, cancer biology, genomics, haematology, oncology, clinical cytogenetics, molecular diagnostics and genetics.

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From the Publisher

This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols ina systematic, easy to follow manner. Because...

Barbara G Beatty is at Health Sciences Research Facility, University of Vermont, USA. Sabine Mai is at Manitoba Institute of Cell Biology, Winnipeg, Canada.

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Format:PaperbackDimensions:274 pages, 9.69 × 7.44 × 0.69 inPublished:May 1, 2002Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199638845

ISBN - 13:9780199638840

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Table of Contents

1. Beatty, Mai, Squire: Introduction2. Bray-Ward: Labelling Methods3. Beatty, Scherer: FISH Mapping4. Mai, Wiener: Murine FISH5. Heng: Fiber FISH6. Tam et al: RNA FISH7. Cremer et al: Three Dimensional FISH8. Lichter, Joos, Schwanen: CGH9. Squire, Marrano, Kolomietz: Clinical Cytogenetics10. Bayani, Squire: Multicolour FISH and SKY11. Khan et al: cDNA Microarrays and FISH

Editorial Reviews

"The book will be of value to researchers and students in molecular pathology, cancer biology, and genomics."--SciTech Book News