Flow Cytometry: A Practical Approach

Paperback | May 18, 2000

EditorMichael Ormerod

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Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a newtechnique. The chapter on immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition ofa chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point ofreference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.

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From the Publisher

Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a newtechnique. The chapter on immunophenotyping -...

Michael Ormerod is a Scientific Consultant, Reigate, Surrey.
Format:PaperbackPublished:May 18, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199638241

ISBN - 13:9780199638246

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Table of Contents

Introduction to the principles of flow cytometryFluorescence and fluorescence technologyPreparing of suspensions of single cellsFlow sortingImmunofluorescence of surface markersAnalysis of DNA - general methodsFurther clinical applicationsQuality control in the clinical laboratoryMeasurement of cytoplasmic and nuclear antigensAnalysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine methodAnalysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide methodChromosome analysis and sorting by flow cytometryIntracellular ionised calcium, magnesium, membrane potential and pHFlow cytometry in the study of apoptosisFurther applications to cell and molecular biology

Editorial Reviews

"This is a great handbook for flow-cytometry laboratories. A big 'plus' is the compilation of well-written protocols for many common flow cytometry based applications in a single conveniently sized and affordable book." -- Doody's