Flow Cytometry For Biotechnology

Paperback | May 10, 2006

EditorLarry A. Sklar

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Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in thelaser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry aversatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant andreproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.

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Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in thelaser beam, on the order of ten microsecond...

Larry Sklar is Regents Professor of Pathology at the University of New Mexico. He is also Director of Basic Research at the University of New Mexico Cancer Research and Treatment Center.

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Format:PaperbackDimensions:400 pages, 6.1 × 9.09 × 0.71 inPublished:May 10, 2006Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0195152344

ISBN - 13:9780195152340

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Table of Contents

Contributors1. Larry A. Sklar: The Future of Flow Cytometry in BiotechnologyPart I. Tools for Flow Cytometry2. Howard M. Shapiro: Fluorescent Probes3. Bruce S. Edwards and Larry A. Sklar: Automation and High Throughput Flow Cytometry4. David Basiji: Multispectral Imaging in Flow: A Technique for Advanced Cellular Studies5. Anne Y. Fu, Yohie Yokobayashi, Frances H. Arnold, and Stephen R. Quake: Elastomeric Microfabricated Fluorescence-Activated Cell Sorters6. Tione Buranda, Larry A. Sklar, and Gabriel P. Lopez: Flow Cytometry, Beads, and Microchannels7. Babetta L. Marrone, Robert C. Habbersett, James H. Jett, Richard A. Keller, Xiaomei Yan, and Thomas M. Yoshida: DNA Fragment Sizing by High-Sensitivity Flow Cytometry: Applications in Bacterial Identification8. Adam Treister: A Guide to Informatics and Flow Cytometry9. Steven W. Graves, John P. Nolan, and Larry A. Sklar: Molecular Assemblies, Probes, and Proteomics in Flow CytometryPart II. Biological Applications10. Mario Roederer and Stephen C. De Rosa: Multiparameter Analysis: Application to Vaccine Analysis11. Erlina Pali, Mark Powell, Esteban Masuda, Yasumichi Hitoshi, Sasha Holland, Mark K. Bennett, Susan M. Molineaux, Donald G. Payan, and James B. Lorens: FACS-based High-Throughput Functional Screening of Genetic Libraries for Drug Target Discovery12. George Georgiou, Barrett R. Harvery, Karl E. Griswold, and Brent L. Iverson: Applications of Flow Cytometry in Protein Engineering13. Duane L. Garner and George E. Seidel, Jr.: Applications of Flow Cytometry in Animal Reproduction14. John P. Nolan, Alina Desphande, and Feng Zhou: Genetic Analysis Using Microsphere Arrays15. Scott W. Burchiel and James L. Weaver: Uses of Flow Cytometry in Preclinical Safety Pharmacology and Toxicology16. David W. Galbraith, Jan Bartos, and Jaroslav Dolozel: Flow Cytometry and Cell Sorting in Plant Biotechnology17. Peter Simons, Charlotte M. Vines, T. Alexander Key, Ross M. Potter, Mei Shi, Larry A. Sklar, Eric R. Prossnitz: Analysis of GTP-Binding Protein-Coupled Receptor Assemblies by Flow Cytometry18. Richard S. Larson, Alexandre Chigaev, Bruce S. Edwards, Sergio A. Ramirez, Stuart S. Winter, Gordon Zwartz, Larry A. Sklar: Applications of Flow Cytometry to Cell Adhesion Biology: From Aggregates to Drug DiscoveryIndex

Editorial Reviews

"Timely and well organized, flow cytometry technology as described here will have a powerful impact on how one approaches high-throughput technologies. This first scholarly treatment of the subject should be read by flow cytometry experts, academic researchers, students, as well as those whoare developing new biotechnology."--Mark J. Soloski, Department of Medicine, The Johns Hopkins University School of Medicine