Fluorescence Spectroscopy and Microscopy: Methods and Protocols by Yves EngelborghsFluorescence Spectroscopy and Microscopy: Methods and Protocols by Yves Engelborghs

Fluorescence Spectroscopy and Microscopy: Methods and Protocols

EditorYves Engelborghs, Antonie J.W.G. Visser

Hardcover | October 10, 2013

Pricing and Purchase Info


Earn 1165 plum® points

In stock online

Ships free on orders over $25

Not available in stores


Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.  The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).  Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both 'novice' and established scientists toward furthering their research with these invaluable techniques.
Title:Fluorescence Spectroscopy and Microscopy: Methods and ProtocolsFormat:HardcoverDimensions:785 pages, 10 × 7.01 × 0.01 inPublished:October 10, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627036482

ISBN - 13:9781627036481

Look for similar items by category:


Table of Contents

Part I: Steady-State Fluorescence Spectroscopy (SSFS) 1. How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra            Adam Matthew Gilmore 2. Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions            Nicholas G. James and David M. Jameson 3. Quantitative Fluorescence Spectral Analysis of Protein Denaturation            Ivo H.M. van Stokkum and Sergey P. Laptenok 4. High-Pressure Fluorescence Applications            Mariano Dellarole and Catherine A. Royer Part II: Time-Resolved Fluorescence Spectroscopy (TRFS) 5. Frequency Domain Fluorometry: Theory and Application            Carissa M. Vetromile and David M. Jameson 6. Polar Plot Representation of Time-Resolved Fluorescence            John Paul Eichorts, Kai wen Teng, and Robert M. Clegg 7. Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics            Tomer Orevi, Eitan Lerner, Gil Rahamim, Dan Amir, and Elisha Haas 8. MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes            Patrik R. Callis and Jose R. Tusell 9. Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins            Dmitri Toptygin 10. Global Analysis of Time-Resolved Fluorescence Data            Anatoli V. Digris, Eugene G. Novikov, Victor V. Skakun, and Vladimir V. Apanasovich 11. Nanometrology            David J.S. Birch and Philip Yip 12. Upconversion Spectrophotofluorometry            Arianna Biesso, Jianhua Xu, and Jay R. Knutson 13. Subpicosecond Kerr-Gate Spectrofluorometry            Sergey P. Laptenok, Patrick Nürnberger, Andras Lukacs, and Marten H. Vos 14. Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay            Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, and Fumio Tanaka Part III: Fluorescent Probe Development (FPD) 15. Biosynthetic Incorporation of Trp Analogs in Proteins            Jaap Broos 16. Optimization of Fluorescent Proteins            Daphne S. Bindels, Joachim Goedhart, Mark A. Hink, Laura van Weeren, Linda Joosen, and Theodorus W.J. Gadella, Jr. 17. Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family            Zeinab Darwich, Andrey S. Klymchenko, and Yves Mély Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP) 18. Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope            Ranhua Xiong, Hendrik Deschout, Jo Demeester, Stefaan C. De Smedt, and Kevin Braeckmans Part V: Fluorescence Microscopy: Förster Resonance Energy Transfer Imaging (FRETim) 19. A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging            Clemens F. Kaminski, Eric. J. Rees, and Gabriele S. Kaminski Schierle Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM) 20. Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors            Roland Hartig, Yury Prokazov, Evgeny Turbin, and Werner Zuschratter 21. Global Analysis of FRET- FLIM Data in Live Plant Cells            Sergey P. Laptenok, Joris J. Snellenburg, Christoph A. Bücherl, Kai R. Konrad, and Jan Willem Borst 22. Time-Resolved Fluorescence Anisotropy Imaging            Klaus Suhling, James Levitt, and Pei-Hua Chung 23. Multi-Modal Fluorescence Imaging Spectroscopy            Martijn H.W. Stopel, Christian Blum, and Vinod Subramaniam Part VII: Fluorescence Microscopy: Fluorescence Fluctuation Spectroscopy (FFS) 24. Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells            Thomas Weidemann 25. Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells            Xiaoxiao Ma, Yong Hwee Foo, and Thorsten Wohland 26. Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS)            Ana M. Melo, Manuel Prieto, and Ana Coutinho 27. PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching            Markus Sauer and Hannes Neuweiler 28. Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes            Tomás Steinberger, Radek Machán, and Martin Hof 29. Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane            Paul Müller, Petra Schwille, and Thomas Weidemann 30. Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS            Jelle Hendrix and Don C. Lamb 31. Quantitative Study of Protein-Protein Interactions in Live Cell by Dual Color Fluorescence Lifetime Cross-Correlation Spectroscopy            Sergi Padilla-Parra, Nicolas Audugé, Maïté Coppey-Moisan, and Marc Tramier 32. Brightness Experiments            Patrick J. Macdonald, Jolene Johnson, Yan Chen, and Joachim D. Mueller 33. Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy            Victor V. Skakun, Anatoli V. Digris, and Vladimir V. Apanasovich 34. Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy            Igor P. Shingaryov, Victor V. Skakun, and Vladimir V. Apanasovich Part VIII: Fluorescence Microscopy: Single-Molecule Fluorescence Spectroscopy (smFS) 35. Single-Molecule Fluorescence of Nucleic Acids            Kaley McCluskey, Euan Shaw, Daniel A. Lafontaine, and J. Carlos Penedo 36. Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Above the Diffraction Limit of Light            Susana Rocha, Herlinde De Keersmaecker, Hiroshi Uji-i, Johan Hofkens, and Hideaki Mizuno