Free Radicals: A Practical Approach by Neville A. PunchardFree Radicals: A Practical Approach by Neville A. Punchard

Free Radicals: A Practical Approach

EditorNeville A. Punchard, Frank J. Kelly

Paperback | April 30, 1999

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Free radicals - reactive, short-lived molecules - are now known to play an important role in a large number of human diseases, including heart disease and cancer, and their study in biological systems has increased enormously in recent years. This volume brings together practical techniquesfor detecting and measuring free radicals, and for studying their effects. It will be of interest to a wide cross-section of biochemists and clinicians.
Neville Punchard is at University of Luton. Frank Kelly is at St Thomas's Hospital, London.
Title:Free Radicals: A Practical ApproachFormat:PaperbackDimensions:334 pages, 9.21 × 6.14 × 0.71 inPublished:April 30, 1999Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635595

ISBN - 13:9780199635597

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Table of Contents

Introduction1. Direct and indirect measurements of free radicals by physico-chemical methods1.1. In vitro and in vivo detection of free radicals metabolites with electron spin resonance1.2. The application of high-field nuclear magnetic resonance spectroscopy to the detection of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs2. Direct and indirect measurement of free radicals by other methods2.1. Intrinsic (low-level) chemiluminescence2.2. Visual assessment of oxidative stress by multifunctional digital microfluorography2.3. Salicylic acid and phenylalanine as probes to detect hydroxyl radicals3. Measurement of free radical products3.1. Lipids (i): peroxides and other products3.2. Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal3.3. Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipid peroxidation3.4. Proteins (i): determination of carbonyl groups in oxidized proteins3.5. Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA3.6. Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems3.7. Nucleic acids: measurement of products of free radical attack on nucleic acids4. Measurement of antioxidants4.1. Glutathione4.2. Glutathione peroxidase: activity and steady-state level of mRBA4.3. Superoxide dismutase4.4. Application of deuterium labelling and gas chromatography-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids4.5. Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants4.6. Measurement of antioxidant gene expressionAppendix

Editorial Reviews

`will greatly assist any laboratory wishing to enter the fray in what must be one of the most exciting areas of medicine, nutritional science and food technology ... the introductory sections to each chapter are in themselves small masterpieces of explanation of the states of the arts forwhich the protocols are relevant. Thus this book will be not just a laboratory manual but should be a bestseller in the student market.'Times Higher Education Supplement