Functional Genomics: A Practical Approach

Paperback | September 15, 2000

EditorStephen Hunt, Frederick . Livesey

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With the complete genomes of many organisms now available, and the first draft of the human genome imminent, there is an increasing demand from researchers in a range of disciplines for techniques that will allow them to utilize these resources in their own research. This book provides acomprehensive treatment of the range of methods available for gene and protein expression profiling in a variety of systems. These include large-scale methods, such as cDNA microarrays, serial analysis of gene expression (SAGE) and proteomics, and also methods suitable for non-specialistlaboratories, such as differential display and suppression subtraction hybridization. In all cases the chapters have been written by the developers of these methods or experienced users and include detailed protocols to facilitate the introduction of these methods to the readers'laboratories.

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With the complete genomes of many organisms now available, and the first draft of the human genome imminent, there is an increasing demand from researchers in a range of disciplines for techniques that will allow them to utilize these resources in their own research. This book provides acomprehensive treatment of the range of methods ...

S. Hunt is at University College London. F. Livesey is at Harvard Medical School.

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Format:PaperbackPublished:September 15, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199637741

ISBN - 13:9780199637744

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Table of Contents

Functional Genomics: approaches and methodologiesConstruction and Screening of a Subtractive cDNA LibraryDifferential Display Analysis of Alterations in Gene ExpressionRepresentational Difference Analysis of cDNAIdentification of Differentially Expressed Genes by Suppression Subtractive Hybridization (SSH)Gene Expression Analysis by cDNA MicroarraysSADE: A Microassay for Serial Analysis of Gene ExpressionAnalysis of Gene Expression in Single Cells Using Three Prime End Amplification (TPEA) PCRAnalysis of Gene Expression by 2-D Gel ElectrophoresisProteome Research: methods for protein characterization

Editorial Reviews

" ... chapters are well organized and the protocols are clearly highlighted ... a good starting point for anybody interested in a 'practical approach' to the topic of gene expression." Trends in Biochemical Sciences