Fundamentals Of Fluorescence Microscopy: Exploring Life With Light by Partha Pratim MondalFundamentals Of Fluorescence Microscopy: Exploring Life With Light by Partha Pratim Mondal

Fundamentals Of Fluorescence Microscopy: Exploring Life With Light

byPartha Pratim Mondal, Alberto Diaspro

Paperback | August 23, 2016

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This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications.  The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Alberto Diaspro, born in Genoa, Italy, on April 7, 1959, received his Laurea in Electronic Engineering in 1983, Univ.of Genoa. Dir. Nanophys at Italian Institute of Technology (IIT), Deputy Director of IIT, Prof. Appl. Phys. at Univ.of Genoa. President of Optics Within Life Sciences, IEEE Senior member and Editor in chief of Microscopy...
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Title:Fundamentals Of Fluorescence Microscopy: Exploring Life With LightFormat:PaperbackDimensions:218 pages, 27.9 × 21 × 0.02 inPublished:August 23, 2016Publisher:Springer NatureLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:940177966X

ISBN - 13:9789401779661

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Table of Contents

Preface.- Acknowledgements.- Dedication.- Ray Optics, Wave Optics and Imaging System Designing.- Basics of Electromagnetic Theory for Fluorescence Microscopy.- Electric Field Effects in Optical Microscopy Systems.- Quantum Description of Radiation Field and Optical Microscopy.- Molecular Physics of Fluorescence Markers.- Basics of Fluorescence and Photophysics.- General Fluorescence Imaging Techniques.- Multiphoton Fluorescence Microscopy.- Super Resolution Fluorescence Microscopy.- Image Reconstruction Methodologies for Fluorescence Microscopy.-  Future Prospective of Fluorescence Microscopy.- Appendix I.- Appendix II.- Appendix III.