Gel Electrophoresis of Proteins: A Practical Approach

Paperback | October 1, 1998

EditorB. D. Hames, D. Rickwood

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This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing,two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillarygel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with copiousadvice to ensure that each method works first time in the reader's hands. The first two editions of Gel Electrophoresis of Proteins: A Practical Approach each gained a strong reputation as easy-to-follow laboratory manuals written by experienced researchers for researchers. The methods werepresented in a clear accessible format and had been fully tested to ensure success in the lab. This new edition will strengthen the reputation of the book still further. It is a 'must have' for all those who currently use gel electrophoresis or who plan to do so.

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From the Publisher

This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing,two-dimensional gel electrophoresis, preparative gel elec...

B. D. Hames is at University of Leeds.
Format:PaperbackPublished:October 1, 1998Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199636400

ISBN - 13:9780199636402

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Table of Contents

1. One-dimensional PAGE2. Protein detection3. Preparative gel electrophoresis4. Capillary SDS electrophoresis5. Conventional isoelectric focusing6. 2-D gel electrophoresis7. Peptide mapping8. Sequence analysis of gel-resolved proteins9. Analysis of glycoproteins10. Protein: protein interactionsAppendix 1 - List of suppliersAppendix 2 - Bibliography of polypeptide detection methods

Editorial Reviews

"A laboratory manual presenting methods in a step-by-step format, and hints and tips from experienced researchers. Among the varieties of gel electrophoresis described are one-dimensional polyacrylamide; isoelectric, two-dimensional; and preparative. Eight of the ten chapters of the earliereditions, in 1981 and 1990, have been rewritten by new authors. They discuss methods that have been developed and tested since then, including capillary gel electrophoresis, the sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analyzing interactionsbetween proteins."--SciTech Book News