Gene Expression Systems: Using Nature for the Art of Expression

Hardcover | November 16, 1998

byJoseph M. FernandezEditorJames P. Hoeffler

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Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Key Features* Provides detailed information on expression systems* Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs* Includes timelines and examples* Compares pros and cons of each method

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Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The...

From the Jacket

Recombinant gene expression is the fastest growing area in the study of molecular biology. By the time the Human Genome Project is completed (Gene Expression Systems covers a variety of promoters and host organisms that researchers can tailor to their specific needs.

Joseph Fernandez is the Vice President of Business Development at Invitrogen Corporation in Carlsbad, California, where he identifies and licenses new technologies including gene expression systems.Jim Hoeffler is the Director of Research and Development at Invitrogen Corporation, concentrating on the development of new products, with ...
Format:HardcoverDimensions:480 pages, 9 × 6 × 0.98 inPublished:November 16, 1998Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0122538404

ISBN - 13:9780122538407

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Table of Contents

Contributors.
Introduction: The Art of Expression.
Purpose of This Book.
Selecting a Suitable Expression System:
Considerations.
Genomics and the Future of Protein Expression Systems,J.M. Fernandez and J.P. Hoeffler.
Prokaryotic Expression Systems:
Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse,R. Durbin.
Expression Vectors Employing thetrcPromoter,J. Brosius.
BacillusExpression: A Gram Positive Model,E. Ferrari and B. Miller.
araBExpression System inEscherichia coli, M. Better.
Eukaryotic Expression Systems:
Adenoviral Vectors for Protein Expression,D.J. von Seggern and G.R. Nemerow.
Expression in the Methalotrophic YeastPichia pastoris, J.M. Cregg.
Recombinant Protein Expression inPichia methanolica, C.K. Raymond.
Cytomegalovirus Promoter for Expression in Mammalian Cells,M.F. Stinski.
Inducible Mammalian Expression Systems,M.Russell.
Protein Expression in Mammalian Cells Using Sindbis Virus,R.P. Bennett.
Expression in Insect Systems:
DrosophilaS2 System for Heterologous Gene Expression,R.B. Kirkpatrick and A. Shatzman.
Baculovirus Expression Vector System,M. Galleno and A.J. Sick.
Transgenic Expression:
Recombinant Protein Expression in Transgenic Mice,R. Abbud and J.H. Nilson.
Expression of Recombinant Proteins in the Milk of Transgenic Animals,H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling.
Recombinant Protein Expression in Plants,A.E. Voloudakis, Y. Yin, and R.N. Beachy.
Index.