Gene Knockout Protocols by Martin J. TymmsGene Knockout Protocols by Martin J. Tymms

Gene Knockout Protocols

EditorMartin J. Tymms, Ismail Kola

Paperback | November 10, 2010

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Highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.

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Title:Gene Knockout ProtocolsFormat:PaperbackDimensions:448 pages, 9.02 × 5.98 × 0.07 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617370800

ISBN - 13:9781617370809

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Table of Contents

Overview: Gene Knockouts, Paul J. Hertzog and Ismail Kola. Isolation and Maintenance of Primate Embryonic Stem Cells, Vivienne S. Marshall, Michelle A. Waknitz, and James A. Thomson. Gene Targeting in ES Cells, Thomas M. DeChiara. Manipulating Mouse Embryonic Stem Cells, Lino Tessarollo. Gene Targeting in a Centralized Facility, Louise D. Barnett and Frank Köntgen. The LoxP/CRE System and Genome Modification, Trevor J. Wilson and Ismail Kola. Creation and Use of a Cre Recombinase Transgenic Database, Andras Nagy and Lynn Mar. Choice of Mouse Strains for Gene Targeting, Mireille H. Lahoud, Trevor J. Wilson, and Paul J. Hertzog. Isolation, Microinjection, and Transfer of Mouse Blastocysts, Angelika Bonin, Susan W. Reid, and Lino Tessarollo. Aggregation Chimeras: Combining ES Cells, Diploid and Tetraploid Embryos, Mika Tanaka, Anna-Katerina Hadjantonakis, and Andras Nagy. How to Study Pathologic Phenotypes of Knockout Mice, Roderick T. Bronson. Analysis of Hematopoietic Phenotypes in Knockout Mouse Models, Ivan Bertoncello and Brenda Williams. Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function, Paul J. Hertzog. Influence of Genetic Background on Knockout Mouse Phenotypes, L. Philip Sanford, Suhas Kallapur, Ilona Ormsby, and Thomas Doetschman. Lineage Allocation During Early Embryogenesis: Mapping of the Neural Primordia and Application to the Analysis of Mouse Mutants, Gabriel A. Quinlan, Bruce P. Davidson, and Patrick P. L. Tam. Generation of Double-Knockout Embryonic Stem Cells, Hein te Riele, Conny Brouwers, and Marleen Dekker. In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes, Anna M. Wobus, Kaomei Guan, and Uta Pich. Embryonic Stem Cells in the Study of Hematopoiesis, Francesca Lazner, Ismail Kola, Elizabeth Stadler, and Andrew G. Elefanty. Interferon-Inducible ES Cell Expression Systems, Linda M. Whyatt and Peter D. Rathjen. Transgenic Studies in the Mouse: Improving the Technology Towards a Conditional Temporal and Spatial Approach, Sika Ristevski. In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping, Desmond J. Smith. Epigenetic Effects on Transgene Expression, Emma Whitelaw, Heidi Sutherland, Margot Kearns, Hugh Morgan, Linda Weaving, and David Garrick. Positional-Candidate Cloning of Genes from Mouse Mutants, Karen B. Avraham. Genetically Engineered Mice: Husbandry and Resources, John J. Sharp, Carol C. Linder, and Larry E. Mobraaten. Embryo Cryopreservation for Transgenic Mouse Lines, Jillian M. Shaw and Magosaburo Kasai. Index.

Editorial Reviews

"This is a valuable reference work, which will no doubt find a home on many lab benches." -E-STREAMS