Gene Probes 1: A Practical Approach

Paperback | March 1, 1992

EditorB. D. Hames, S. J. Higgins

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The preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuseson the wide range of techniques now available for the purification, synthesis, labelling, detection, and quantitation of probes, including antibody probes. Gene Probes 2 will concentrate on describing techniques for specific research goals. Contents List: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of oligodeoxynucleotide probes; Probe preparation by PCR; Synthesis of labelled riboprobes; Antibody probes

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The preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuseson the wide range of techniques now availabl...

S. J. Higgins is at University of Leeds.

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Format:PaperbackPublished:March 1, 1992Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199634009

ISBN - 13:9780199634002

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Table of Contents

Introduction1. Strategies for using gene probes2. Preparation of nucleic acids for use as probes3. DNA radiolabelling and detection4. Non-radioactive labelling of DNA probes5. Preparation of synthetic oligodeoxynucleotide probes6. Probe preparation of PCR7. Synthesis of labelled riboprobes8. Antibody probesAppendix 1Appendix 2

Editorial Reviews

`a very practical book'Cellular and Molecular Biology, Vol. 42, No. 3, 1996