Gene Probes 2: A Practical Approach by B. D. Hames

Gene Probes 2: A Practical Approach

EditorB. D. Hames, S. J. Higgins

Paperback | March 1, 1992

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Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to analysis of human genetic mutations. ACompanion volume, Genes Probes 1, covers preparation of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance.

About The Author

S. J. Higgins is at University of Leeds.
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Title:Gene Probes 2: A Practical ApproachFormat:PaperbackPublished:March 1, 1992Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199634149

ISBN - 13:9780199634149

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Table of Contents

1. Hybridization strategy2. Screening recombinant DNA libraries by hybridization and amplification3. Screening human recombinant libraries with nucleic acid probes4. Antibody screening of bacterial expression systems5. Isolation of cDNA clones by direct expression in mammalian cells6. Genomic mapping and gene structure analysis7. Analysis of gene expression by blotting techniques8. In situ hybridization of nucleic acid probes to cellular RNA9. Applications of chromosomal in situ hybridization10. Analysis of gene expression analysis by PCR: a quantitative approach11. Analysis of protein expression by immunocytochemistry12. Gene targeting in mammalian cells13. Directed mutagenesis and mutant analysis14. Human gene mutational analysisAppendix 1Appendix 2