Gene Targeting: A Practical Approach by Alexandra Joyner

Gene Targeting: A Practical Approach

EditorAlexandra Joyner

Paperback | December 9, 1999

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Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practicalprotocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells.The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using theCre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simplemethod for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trapstrategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidategene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.

About The Author

Alexandra Joyner is at HHMi and Skirball Institute of Biomolecular Medicine, NYU Medical Centre.

Details & Specs

Title:Gene Targeting: A Practical ApproachFormat:PaperbackDimensions:312 pages, 9.21 × 6.14 × 0.71 inPublished:December 9, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:019963792x

ISBN - 13:9780199637928

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Table of Contents

Preface1. Bradley et al: Gene targeting vectors for mammalian cells2. Dymecki: Site specific recombination in cell and mice3. Matise and Joyner: Production of targeted ES cell clones4. Papaioannou and Johnson: Production of chimeras and genetically defined offspring from targeted ES cells5. Nagy and Rossant: Production of completely ES cell-derived fetuses6. Wurst and Gossler: Gene and enhancer traps in ES cells7. Bultman and Magnuson: Mouse genetics and analysis of mutant phenotypesIndex

Editorial Reviews

" ... a collection of state-of-the-art protocols" Cambridge Scientific Abstracts