Genomic Imprinting: Methods and Protocols by Andrew WardGenomic Imprinting: Methods and Protocols by Andrew Ward

Genomic Imprinting: Methods and Protocols

EditorAndrew Ward

Paperback | November 10, 2010

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Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. Protocols are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and methylation-sensitive DNA-binding proteins, and for examining chromatin structure. There are also methods for the manipulation of mouse embryos to produce monoparental embryos and embryonic stem cells, and for the generation of transgenic mice with BAC, PAC,and YAC constructs. Each technique is described in step-by-step detail to ensure successful results.
Title:Genomic Imprinting: Methods and ProtocolsFormat:PaperbackDimensions:390 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371645

ISBN - 13:9781617371646

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Table of Contents

Generation of Monoparental Embryos for Investigation into Genomic ImprintingWendy L. Dean, Gavin Kelsey, and Wolf ReikDeriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic ImprintingJeffrey R. MannBalanced Translocations for the Analysis of Imprinted Regions of the Mouse GenomeAnne C. Ferguson-Smith, Maxine Tevendale, Pantelis Georgiades, and Valerie GrandjeanProduction of YAC Transgenic Mice by Pronuclear InjectionJustin F.-X. Ainscough, Rosalind M. John, and Sheila C. BartonA Transgenic Approach to Studying Imprinted Genes: Modified BACs and PACsRosalind M. John, Justin F.-X. Ainscough, and Sheila C. BartonMethylation-Sensitive Genome ScanningIzuho Hatada and Tsunehiro MukaiSubtraction-Hybridization Method for the Identification of Imprinted GenesFumitoshi Ishino, Yoshimi Kuroiwa, Naoki Miyoshi, Shin Kobayashi, Takashi Kohda, and Tomoko Kaneko-IshinoIdentification of Imprinted Loci by Methylation: Use of Methylation- Sensitive Representational Difference Analysis (Me-RDA)Rachel J. Smith and Gavin KelseyRibonuclease ProtectionJoanne L. Thorvaldsen and Marisa S. BartolomeiQuantitative RT-PCR-Based Analysis of Allele-Specific Gene ExpressionJudith Singer-Sam and Chunguang GaoAllele-Specific In Situ Hybridization (ASISH)Rolf Ohlsson, Kristian Svensson, Hengmi Cui, Helena Malmikumpu, and Gail AdamRNA-FISH to Analyze Allele-Specific ExpressionGiovanna BraidottiFlow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted ChromosomesJanine LaSalle and Marc LalandeSouthern Analysis Using Methyl-Sensitive Restriction EnzymesTom MooreA PCR-Based Method for Studying DNA MethylationMira ArielBisulfite-Based Methylation Analysis of Imprinted GenesSabine Engemann, Osman El-Maarri, Petra Hajkova, Joachim Oswald, and Joern WalterDirect Analysis of Chromosome MethylationDéborah Bourc'his and Evani Viegas-PéquignotIn Vitro Methylation of Predetermined Regions in Recombinant DNA ConstructsIlse Van den Wyngaert, Roger L. P. Adams, and Stefan U. KassIn Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and ReligationGhislaine Dell, Marika Charalambous, and Andrew WardDetection of Methyl-Sensitive DNA-Binding Proteins with Possible Involvement in the Imprinting PhenomenonKerstin Otte and Björn RozellProbing Chromatin Structure with Nuclease Sensitivity AssaysRichard I. Gregory, Sanjeev Khosla, and Robert FeilExamining Histone Acetylation at Specific Genomic RegionsJi-Fan Hu and Andrew R. HoffmanPurification of the MeCP2/Histone Deacetylase Complex from Xenopus laevisPeter L. Jones, Paul A. Wade, and Alan P. WolffeReconstitution of Chromatin In VitroKiyoe Ura and Yasufumi KanedaGenomic Imprinting in PlantsRinke Vinkenoog, Melissa Spielman, Sally Adams, Hugh G. Dickinson, and Rod J. Scott

Editorial Reviews

"...a multi-authored volume in which the methods of gene imprinting are discussed in detail in 25 chapters. The protocols are very detailed with multiple schemes and figures." - Journal of Pediatric Endocrinology and Metabolism