Genotoxicity Assessment: Methods and Protocols by Alok DhawanGenotoxicity Assessment: Methods and Protocols by Alok Dhawan

Genotoxicity Assessment: Methods and Protocols

EditorAlok Dhawan, Mahima Bajpayee

Hardcover | July 30, 2013

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Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In Genotoxicity Assessment: Methods and Protocols, expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques, and primary DNA damage, assays in alternate to animal models, and updated ICH guidelines. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Genotoxicity Assessment: Methods and Protocols seeks to aid research students and scientists working in regulatory toxicology as well as biomedical, biochemical and pharmaceutical sciences.
Title:Genotoxicity Assessment: Methods and ProtocolsFormat:HardcoverDimensions:430 pages, 10 × 7.01 × 0.1 inPublished:July 30, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627035281

ISBN - 13:9781627035286

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Table of Contents

Part I: Gene Mutation Assays1. Bacterial Mutation AssaysErrol Zeiger  2. In Vitro Mouse Lymphoma (L5178Y Tk+/- -3.7.2.C) Forward Mutation AssayMelissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi 3. Erythrocyte--Based Pig--a Gene Mutation AssayJeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger 4. Detection of in vivo Mutation in the Hprt and Pig--a Genes of Rat LymphocytesVasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich 5. In vivo cII, gpt, and Spi- gene Mutation Assays in Transgenic Mice and RatsMugimane G. Manjanatha, Xuefei Cao, Sharon D. Shelton, Roberta A. Mittelstaedt, and Robert H. Heflich Part II; Assays for Chromosomal Abnormalities6. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests Pasquale Mosesso, Serena Cinelli, Adyapalam T. Natarajan, and Fabrizio Palitti 7. Analysis of Chromosome Aberrations in Somatic and Germ Cells of the MouseFrancesca Pacchierotti and Valentina Stocchi 8. Chromosomal Aberration Test in Human LymphocytesChristian Johannes and Guenter Obe 9. In vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral BloodSawako Kasamoto, Shoji Masumori, and Makoto Hayashi 10. Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells Claudia Bolognesi and Michael Fenech 11. Flow Cytometric Determination of Micronucleus FrequencyAzeddine Elhajouji and Magdalena Lukamowicz-Rajska 12. Fluorescent in situ Hybridization (FISH) Technique for the Micronucleus TestIlse Decordier and Micheline Kirsch-Volders 13. Comparative Genomic Hybridization (CGH) GenotoxicologyBaumgartner A14. The in vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and CytotoxicityBella B. Manshian, Neenu Singh, and Shareen H. Doak Part III: Tests for Primary DNA Damage15. g--H2AX Detection in Somatic and Germ Cells of MiceEugenia Cordelli and Lorena Paris 16. Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response BiomarkersJulian Laubenthal, Michal R. Gdula , Alok Dhawan, and Diana Anderson 17. The Comet Assay: Assessment of in vitro and in vivo DNA DamageMahima Bajpayee, Ashutosh Kumar, and Alok Dhawan 18. The Comet Assay in Human BiomonitoringDiana Anderson, Alok Dhawan, and Julian Laubenthal 19. The Comet assay in Marine AnimalsG. Frenzilli and B.P. Lyons 20. Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivoFabrice Nesslany 21. 32P--Postlabeling Analysis of DNA AdductsHeinz H. Schmeiser, Marie Stiborova, and Volker M. Arlt Part IV: Assays in Plants and Aleternate Animal Models22. Micronucleus Assay with Tetrad Cells of TradescantiaMiroslav Misík, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, and Siegfried Knasmueller 23. The Wing--Spot and the Comet Tests as useful Assays Detecting Genotoxicity in DrosophilaRicard Marcos and Erico R. Carmona Part V: Guidelines24. Genotoxicity Guidelines Recommended by International Conference of Harmonization (ICH)Gireesh Kamath H. and K.S. Rao