Guide To Yeast Genetics And Molecular Biology: Volume 194: Guide To Yeast Genetics And Molecular…

Other | May 1, 2004

byAbelson, John N., John N. Abelson

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Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast.

Key Features
* Basic Methods in Yeast Genetics
* Physical and genetic mapping
* Making and recovering mutants
* Cloning and Recombinant DNA Methods
* High-efficiency transformation
* Preparation of yeast artificial chromosome vectors
* Basic Methods of Cell Biology
* Immunomicroscopy
* Protein targeting assays
* Biochemistry of Gene Expression
* Vectors for regulated expression
* Isolation of labeled and unlabeled DNA, RNA, and protein

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From the Publisher

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume co...

Format:OtherDimensions:933 pages, 1 × 1 × 1 inPublished:May 1, 2004Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0080575137

ISBN - 13:9780080575131


Extra Content

Table of Contents

Basic Methods of Yeast Genetics:
F. Sherman, Getting Started with Yeast.
F. Sherman and J. Hicks, Micromanipulation and Dissection of Asci.
F. Sherman and P. Wakem, Mapping Yeast Genes.
S.L. Gerring, C. Connelly, and P. Hieter, Positional Mapping of Genes by Chromosome Blotting and Chromosome Fragmentation.
G.F. Sprague, Jr., Assay of Yeast Mating Reaction.
Y. Kassir and G. Simchen, Monitoring Meiosis and Sporulation inSaccharomyces cerevisiae.
R.E. Esposito, M. Dresser, and M. Breitenbach, Identifying Sporulation Genes, Visualizing Synaptonemal Complexes, and Large-Scale Spore and Spore Wall Purification.
I. Herskowitz and R.E. Jensen, Putting theHOGene to Work: Practical Uses for Mating-Type Switching.
B. Rockmill, E.J. Lambie, and G.S. Roeder, Spore Enrichment.
T.D. Fox, L.S. Folley, J.J. Mulero, T.W. McMullin, P.E. Thorsness, L.O. Hedin, and M.C. Costanzo, Analysis and Manipulation of Yeast Mitochondrial Genes.
Cloning and Recombinant DNA:
P. Philippsen, A. Stotz, and C. Scherf, DNA ofSaccharomyces cerevisiae.
D.M. Becker and L. Guarente, High-Efficiency Transformation of Yeast by Electroporation.
J.A. Heinemann and G.F. Sprague, Jr., Transmission of Plasmid DNA to Yeast by Conjugation with Bacteria.
M.D. Rose and J.R. Broach, Cloning Genes by Complementation in Yeast.
R.A. Young and R.W. Davis, Gene Isolation with
J. Rine, Gene Overexpression in Studies ofSaccharomyces cerevisiae.
D.T. Burke and M.V. Olson, Preparation of Clone Libraries in Yeast Artificial-Chromosome Vectors.
Making Mutants:
C.W. Lawrence, Classical Mutagenesis Techniques.
R. Rothstein, Targeting, Disruption, Replacement, and Allele Rescue. Integrative DNA Transformation in Yeast.
R.S. Sikorski and J.D. Boeke,In VitroMutagenesis and Plasmid Shuffling.
From Cloned Gene to Mutant Yeast:
J.N. Strathern and D.R. Higgins, Recovery of Plasmids from Yeast intoEscherichia coli.
M.F. Hoekstra, Shuttle Vectors.
H.S. Seifert, J. Nickoloff, and F. Heffron, Shuttle Mutagenesis.Bacterial Transposons for Genetic Manipulations in Yeast:
D.J. Garfinkel and J.N. Strathern, Ty Mutagenesis inSaccharomyces cerevisiae.
R.P. Moerschell, G. Das, and F. Sherman, Transformation of Yeast Directly with Synthetic Oligonucleotides.
Biochemistry of Gene Expression:
J.C. Schneider and L. Guarente, Vectors for Expression of Cloned Genes in Yeast.
Regulation, Overproduction, and Underproduction:
M. Schena, D. Picard, and K.R. Yamamoto, Vectors for Constitutive and Inducible Gene Expression in Yeast.
K. Kadohrer and H. Domdey, Preparation of High Molecular Weight RNA.
J.A. Wise, Preparation and Analysis of Low Molecular Weight RNAs and Small Ribonucleoproteins.
R. Parker, D. Herrick, S.W. Peltz, and A. Jacobson, Measurement of mRNA Decay Rates inSaccharomyces cerevisiae
J.R. Warner, Labeling of RNA and Phosphoproteins inSaccharomyces cerevisiae.
E.W. Jones, Tackling the Protease Problem inSaccharomyces cerevisiae.
H.A. Rauaae, W.H. Mager, and R.J. Planta, Structural and Functional Analysis of Yeast Ribosomal Proteins.
T.J. Koerner, J.E. Hill, A.M. Myers, and A. Tzagoloff, High-Expression Vectors with Multiple Cloning Sites for Construction of trpE Fusion Genes: pATH Vectors.
D.T. Moir and L.S. Davidow, Production of Proteins by Secretion from Yeast.
P.A. Kolodziej and R.A. Young, Epitope Tagging and Protein Surveillance.
K. Struhl, Reverse Biochemistry: Methods and Applications for Synthesizing Yeast Proteinsin Vitro
M.J. Leibowitz, F.P. Barbone, and D.E. Georgopoulos,In VitroProtein Synthesis.
N.F. Lue, P.M. Flanagan, R.J. Kelleher III, A.M. Edwards, and R.D. Kornberg, RNA Polymerase II Transcriptionin Vitro.
J.M. Huibregtse and D.R. Engelke, Direct Sequence and Footprint Analysis of Yeast DNA by Primer Extension.
Cell Biology:
J.R. Pringle, A.E.M. Adams, D.G. Drubin, and B.K. Haarer, Immunofluorescence Methods for Yeast.
B. Byers and L. Goetsch, Preparation of Yeast Cells for Thin-Section Electron Microscopy.
M.W. Clark, Immunogold Labeling of Yeast Ultrathin Sections.
M.P. Yaffe, Analysis of Mitochondrial Function and Assembly.
C.J. Roberts, C.K. Raymond, C.T. Yamashiro, and T.H. Stevens, Methods for Studying the Yeast Vacuole.
A. Franzusoff, J. Rothblatt, and R. Schekman, Analysis of Polypeptide Transit through Yeast Secretory Pathway.
P.D. Garcia, W. Hansen, and P. Walter,In VitroProtein Translocation across Microsomal Membranes ofSaccharomyces cerevisiae.
P. Orlean, M.J. Kuranda, and C.F. Albright, Analysis of Glycoproteins fromSaccharomyces cerevisiae.
V. Dulic, M. Egerton, I. Elguindi, S. Raths, B. Singer, and H. Riezman, Yeast Endocytosis Assays.
C.M. Nicolet and E.A. Craig, Inducing and Assaying Heat-Shock Response inSaccharomyces cerevisiae.
M.W. Clark, Nucleolar-Specific Positive Stains for Optical and Electron Microscopy.
A.E.M. Adams and J.R. Pringle, Staining of Actin with Fluorochrome-Conjugated Phalloidin.
J.R. Pringle, Staining of Bud Scars and Other Cell Wall Chitin with Calcofluor.
J.P. Aris and G. Blobel, Isolation of Yeast Nuclei.
J.H. Shero, M. Koval, F. Spencer, R.E. Palmer, P. Hieter, and D. Koshland, Analysis of Chromosome Segregation inSaccharomyces cerevisiae.
V. Berlin, J.A. Brill, J. Trueheart, J.D. Boeke, and G.R. Fink, Genetic Screens and Selections for Cell and Nuclear Fusion Mutants.
Fission Yeast:
S. Moreno, A. Klar, and P. Nurse, Molecular Genetic Analysis of Fission YeastSchizosaccharomyces pombe.
R.K. Mortimer, D. Schild, C.R. Contopoulou, and J.A. Kans, Genetic and Physical Maps ofSaccharomyces cerevisiae.
Each chapter includes references.
Author Index.
Subject Index.