Guidelines for Molecular Analysis in Archive Tissues by Giorgio StantaGuidelines for Molecular Analysis in Archive Tissues by Giorgio Stanta

Guidelines for Molecular Analysis in Archive Tissues

EditorGiorgio Stanta

Hardcover | June 27, 2011

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A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.
Giorgio Stanta is Professor of Pathology at the University of Trieste. For many years his main interest has been the application of molecular analysis to fixed and paraffin-embedded tissues (all human tissues of biopsy or surgical origin), called "archive tissues". This research line started thanks to his collaboration with the researc...
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Title:Guidelines for Molecular Analysis in Archive TissuesFormat:HardcoverDimensions:339 pages, 10.24 × 7.6 × 0 inPublished:June 27, 2011Publisher:Springer Berlin HeidelbergLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:3642178898

ISBN - 13:9783642178894

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Table of Contents

Introduction.- Archive Tissues.- Pre-Analytical Treatment of Tissues.- Dissection of Tissue Components.- Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids.- Reverse Transcription (RT).- Nucleic Acid Amplification.- DNA Sequencing.- Microsatellite Analysis.- Methylation Analysis.- Analysis of Copy Number Variations.- Microarrays.- Protein Extraction in Archive Tissues.- Protein Electrophoretic Separation.- Protein Immunodetection.- Mass Spectrometry.- Elements of Good Laboratory Practice..- DNA Sequencing.- Microsatellite Analysis.- Methylation Analysis.- Analysis of Copy Number Variations.- Microarrays.- Protein Extraction in Archive Tissues.- Protein Electrophoretic Separation.- Protein Immunodetection.- Mass Spectrometry.- Elements of Good Laboratory Practice.

Editorial Reviews

From the reviews:"This is a valuable resource that provides precise guidelines for molecular analysis of archive tissues essential for pathologists and researchers involved in translational research and diagnostics. . students, laboratory technicians, and physician-scientists interested in molecular analysis of archive tissues will find this book useful. . The clear explanations should enable even less experienced researchers to follow the protocols."­­­ (Omer Iqbal, Doody's Review Service, December, 2011)