Haemophilus influenzae Protocols by Mark A. HerbertHaemophilus influenzae Protocols by Mark A. Herbert

Haemophilus influenzae Protocols

EditorMark A. Herbert, Derek W. Hood, E. Richard Moxon

Paperback | November 9, 2010

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In HaemophilusiInfluenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of H. influenzae. Described with step-by-instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. These methods illuminate how the bacterium causes disease, as well as how best to develop novel vaccines and antibiotics against the organism.
Title:Haemophilus influenzae ProtocolsFormat:PaperbackDimensions:345 pages, 9.25 × 6.1 × 0 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372552

ISBN - 13:9781617372551

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Table of Contents

The Pathogenesis of Disease Due to Nontypeable Haemophilus influenzaeGail G. Hardy, Simone M. Tudor, and Joseph W. St. Geme, III.The Pathogenesis of Disease Due to Type b Haemophilus influenzaeRuth Aubrey and Christoph TangGeneral Methods for Culturing Haemophilus influenzaeGrant Poje and Rosemary J. RedfieldTransformation of Haemophilus influenzaeGrant Poje and Rosemary J. RedfieldDiagnosis of InfectionAlex van Belkum and Loek van AlphenCharacterization of PlasmidsIoanna D. Dimopoulou, Mohd-Zain Zaini, and Derrick W. CrookProtein Characterization by Two-Dimensional Gel ElectrophoresisPhillip Cash and J. Simon KrollMonitoring Gene Expression Using DNA ArraysTahir R. Ali, Ming-Shi Li, and Paul R. LangfordGene Expression TechnologyXavier de Bolle and Christopher D. BaylissThe Genome Sequence of Haemophilus influenzaeDerek W. HoodStructural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzaeElke K. H. Schweda and James C. RichardsMutagenesis of H. influenzaeMark A. HerbertTransposon Tn10Stefan Schlör, Gabriele Kemmer, and Joachim ReidlIn Vivo Expression of Bacterial Genes During Human InfectionsMartin Handfield, Trevor Seifert, and Jeffrey D. HillmanELISAJoyce S. Plested, Philip A. Coull, and Margaret Anne J. GidneyOpsonophagocytosis Assay Using Flow-CytometryJoyce S. Plested and Philip A. CoullIn Vitro Models of Infection I-Human Respiratory Tissue Organ CultureAndrew M. Middleton, Paula Keig, and Robert Wilsonn Vitro Models of Infection II-Human Umbilical Vein Endothelial Cells (HUVECs) SystemMumtaz Virji and Darryl J. HillAnimal ModelsIndex

Editorial Reviews

"This excellent book is recommended for investigators involved in laboratory research on H. influenzae from the beginning student to the experienced investigator. The book contains abundant, detailed protocols and practical tips that will be useful for all investigators." - Clinical Infectious Diseases"...an excellent source book and an inspiring reading." - International Journal of Medical Microbiology "In addition to detailed information provided in the individual chapters, the book is also valuable by providing an extensive list of 841 references....there are not many microbiological books of such high quality. The authors present highly specialized information in a comprehensible and friendly way." -Folia Microbioligica