Haemopoiesis: A Practical Approach: A Practical Approach

Paperback | May 1, 1994

EditorN. G. Testa, G. Molineux

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The cells and regulators involved in haemopoiesis are well characterized, providing biochemists and cell and molecular biologists with an exciting system in which to study normal and abnormal growth and differentiation. Rapid progress has also been made in the application of researchtechniques to human cells, giving rise to new therapeutic approaches to diseases such as leukaemia. This is a detailed guide to the most important methods in use in this area today, and will be of major interest to haematologists and researchers in many areas of basic and medical research.

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The cells and regulators involved in haemopoiesis are well characterized, providing biochemists and cell and molecular biologists with an exciting system in which to study normal and abnormal growth and differentiation. Rapid progress has also been made in the application of researchtechniques to human cells, giving rise to new therape...

G. Molineux, both at the Paterson Institute for Cancer Research, Manchester.

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Format:PaperbackDimensions:318 pagesPublished:May 1, 1994Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199633657

ISBN - 13:9780199633654

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Table of Contents

1. B.I. Lord: In vivo assays for multipotential and marrow repopulating cells2. B.I. Lord, and J.C.W. Marsh: Enrichment of progenitor cell populations from murine and human haemopoietic tissues3. C.M. Heyworth and E. Spooncer: In vitro clonal assays for murine multipotential and lineage restricted myeloid progenitor cells4. E. Spooncer, J. Eliason, and T.M. Dexter: Long-term mouse bone marrow cultures5. L.H. Coutinho, M.H. Gilleece, E.A. de Wynter, A. Will, and N.G. Testa: Clonal and long-term cultures using human bone marrow6. E. Spooncer, D. Boettiger, and T.M. Dexter: Isolation and culture of factor-dependent haemopoietic cell lines7. Development of haemopoietic cells in liquid culture8. F. Walker and C.M. Heyworth: Analysis of growth factor binding to haemopoietic cells9. P.J. Owen, C.A. Evans, and A.D. Whetton: Signal transduction10. L.J. Fairbairn, and E. Spooncer: Retroviral gene transfer into haemopoietic cells11. A.J. Friedenstein, E. Luria, and G. Molineux: Assays of the haemopoietic microenvironment12. T.D. Allen, and E.A. de Wynter: The visualization of haemopoietic and stromal cell types13. L.H. Coutinho, J. Chang, N.G. Testa, and T.M. Dexter: Autologous bone marrow transplantation using cultured marrow cells in patients with leukaemia14. Analysis of gene expression in haemopoietic cells15. J.C.W. Marsh, I.N. Hampson, and G.J. Cowling: Molecular analysis of clonality and transplantation in haemopoiesis

Editorial Reviews

`The tried formula of technical chapters with very detailed recipes and extensive practical advice provide the information never published in any materials and methods sections ... a continued high level of quality is maintained ... Many of the techniques are pertinent to anyone working withcells in suspension in other fields of biological research and as such the book, especially at the price asked, should be attractive to many workers and laboratories active in other fields of research.'Cell Biology International, Vol. 18, No. 2, 1994