Haemostasis: Methods And Protocols by Paul MonagleHaemostasis: Methods And Protocols by Paul Monagle

Haemostasis: Methods And Protocols

byPaul Monagle

Paperback | August 23, 2016

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The haemostatic system is one the most important physiological systems for maintaining health and well being, and thus the investigation of the haemostatic system remains a research priority. Disturbances of the haemostatic system in the broader sense, such as heart disease and strokes, arguably constitute the single greatest contribution to non-infectious mortality in the world today. Therefore, understanding the laboratory methods to assess the haemostatic system is vital for the practice of complex clinical medicine.InHaemostasis: Methods and Protocols, experts in the field address the major components of the haemostatic system, general principles of haemostatic testing, and techniques used to assess various aspects of the haemostatic system, grouped according to their functional indications. Written in the successfulMethods in Molecular Biology'¢series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible,Haemostasis: Methods and Protocolsprovides an ideal guide to scientists of all backgrounds and serves an urgent need for further research to develop superior methods of assessing the haemostatic system in humans.

Title:Haemostasis: Methods And ProtocolsFormat:PaperbackDimensions:431 pagesPublished:August 23, 2016Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1493961020

ISBN - 13:9781493961023


Table of Contents

Part I. The Haemostatic System

1. The Coagulation System in Humans

Anthony K. C. Chan and Nethnapha Paredes

2. Platelet Physiology

Matthew D. Linden

3. The Role of the Blood Vessel Wall

Nethnapha Paredes and Anthony K. C. Chan

Part II. General Principles of Haemostatic Testing

4. Specimen Requirements for the Haemostasis Laboratory

Linda Stang and Lesley G. Mitchell

5. Principles of Haemostasis Tests

Gemma Crighton

6. Reference Ranges of Coagulation Tests

Oliver Speer, Markus Schmugge, Claudia Metzger, and Manuela Albisetti

7. Lupus Anticoagulant Testing

Karen Moffat, Anne Raby, and Mark Crowther

Part III. Examples of Global Tests of Haemostasis

8. Activated Partial Thromboplastin Time (APTT)

Vera Ignjatovic

9. Prothrombin Time (PT) and International Normalised Ratio (INR)

Vera Ignjatovic

10. Thrombin Clotting Time (TCT)

Vera Ignjatovic

11. Thrombin Generation

Leslie R. Berry and Anthony K. C. Chan

12. Activated Clotting Time (ACT)

Stephen Horton and Simon Augustin

Part IV. Examples of Tests for Bleeding Disorders

13. Factor XII Assays

Stephen Opat, Jenny Butler, Erica Malan, Elizabeth Duncan, and Huyen A.M. Tran

14. Fibrinogen

Linda Stang and Lesley G. Mitchell

15. Platelet Counting

Sylvain Salignac, Véronique Latger-Cannard, Nicole Schlegel, and Thomas Lecompte

16. Platelet Morphology Analysis

Véronique Latger-Cannard, Odile Fenneteau, Sylvain Salignac, Thomas Lecompte, and Nicole Schlegel

17. Light Transmission Aggregometry

Juan Pablo Frontroth

18. Platelet Flow Cytometry

Matthew D. Linden

Part V. Examples of Specific Assays Related to Anticoagulant Activity

19. Anti-factor Xa assay (anti-Xa)

Fiona Newall

20. Reptilase Time (RT)

Hratsch Karapetian

21. Protamine Titration

Fiona Newall

22. Laboratory Methods for the Assay if Tissue Factor Pathway Inhibitor (TFPI) in Human Plasma

Robyn Summerhayes

23. Heparin Induced Thrombocytopenia (HIT)

Andreas Greinacher, Birgitt Fürll, and Sixten Selleng

Part VI. Examples of Tests for Inhibitors of Coagulation

24. Nijmegen-Bethesda Assay to Measure Factor VIII Inhibitors

Elizabeth Duncan, Margaret Collecutt, and Alison Street

25. Kaolin Clotting Time

Kottayam Radhakrishnan

26. The Dilute Russell Viper Venom Time (DRVVT)

Kottayam Radhakrishnan

27. Platelet Neutralisation Test

Kottayam Radhakrishnan

Part VII. Examples of Tests for Increased Risk of Thrombosis

28. Antithrombin

Mirta Hepner and Vasiliko Karlaftis

29. Protein C

Mirta Hepner and Vasiliki Karlaftis

30. Protein S

Mirta Hepner and Vasiliki Karlaftis

31. Testing for Hyperhomocysteinemia in Subjects with a History of Thromboembolic Events Using HPLC Technique

Jonas Denecke

32. Anticardiolipin Antibody and Anti-Beta 2 Glycoprotetin I Assays

Anne Raby, Karen Moffat, and Mark Crowther

33. Testing for Apolipoprotein (a) Phenotype Using Isoelectric Focusing and Immunoblotting Technique

Claus Langer, Bertram Tambyrayah, and Ulrike Nowak-Göttl

Part VIII. Examples of Measures of Fibrinolysis

34. D-Dimer and Fibrinogen/Fibrin Degradation Products

Linda Stang