Handbook of Single-Molecule Biophysics

Hardcover | July 24, 2009

EditorPeter Hinterdorfer, Antoine van Oijen

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This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

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This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and b...

From the Jacket

The last decade has seen the development of a number of novel biophysical methods that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at this fundamental level of sensitivity has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal ...

Format:HardcoverDimensions:672 pages, 9.25 × 6.1 × 0.04 inPublished:July 24, 2009Publisher:Springer New YorkLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0387764968

ISBN - 13:9780387764962

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Table of Contents

Chapter 1 - Single-molecule fluorescence trackingAhmet Yildiz (UCSF)Chapter 2 - Single molecule studies in the membraneGerhard Schuetz (Linz)Thomas Schmidt (Leiden)Chapter 3 - Single-molecule imaging in live cellsJie Xiao (Johns Hopkins)Chapter 4 - Superresolution imagingXiaowei Zhuang (Harvard)Chapter 5 - Fluorescence Resonance Energy TransferAchillefs Kapanidis (Oxford) Chapter 6 - Single-molecule enzymologyAntoine van Oijen (Harvard)Chapter 7 - Rotary proteinsRichard Berry (Oxford) Chapter 8 - Fluorescence Correlation SpectroscopyPetra Schwille (Dresden) Chapter 9 - Advanced Concepts of Fluorescence Fluctuation SpectroscopyJörg Enderlein (Tübingen) Chapter 10 - Nucleic-acid analysis at the single-molecule levelAmit Meller (Boston University)Chapter 11 - Nanopores: Geneneration and Single-Molecule ApplicationsStefan Howorka (UCL)Zuzanna Siwy (UC-Irvine)Chapter 12 - Optical trappingMichael Woodside (Alberta)Megan Valentine (UCSB)Chapter 13 - Magnetic tweezingNynke Dekker (Delft)Chapter 14 - Protein/DNA unfoldingMatthias Rief (Munich) Chapter 15 - Single molecule recognitionZiv Reich (Weizman)Peter Hinterdorfer (Linz)Chapter 16 - Nano-scale AFM imagingSimon Scheuring (Institut Curie)Daniel J. Müller (Dresden)Chapter 17 - High Speed AFMT. Ando (Kanazawa) Chapter 18 - Recognition imagingYves Dufrene (Leuven) Peter Hinterdorfer (Linz)Chapter 19 - Atomic force microscopy of protein-protein interactionsVincent Moy (Miami) Chapter 20 - A New Approach to Analysis of Single Molecule Force MeasurementsEvan Evans (Vancouver) Chapter 21 - Single Molecule Recognition: Extracting Information from Individual Binding Events and Their CorrelationCheng Zhu (Georgia Tech)

Editorial Reviews

From the reviews:"The Handbook of Single-Molecule Biophysics, edited by Hinterdorfer (Johannes Kepler Univ. of Linz, Austria) and van Oijen (Harvard Medical School), contains 21 chapters that address various aspects of techniques used to investigate single-molecule biophysics. The chapters, many authored by leading experts in their respective fields, cover specialized biophysical techniques, their applications, background into the technical design, and practical aspects of implementing these approaches. . Summing Up: Recommended. Graduate students, researchers, faculty, and professionals." (C. A. Reinhart-King, Choice, Vol. 47 (10), June, 2010)