Handbook of Single Molecule Fluorescence Spectroscopy

Paperback | September 1, 2013

byChris Gell, David Brockwell, Alastair Smith

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Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, andphotophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications inbiophysics. It is ideal for graduate research students and others embarking on work in this exciting field.

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From the Publisher

Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, andphotophysics provides a rich playground for mole...

Chris Gell obtained his MPhys. in Physics with Electronics and Instrumentation in 1998 from the University of Leeds. He then obtained his PhD in 2001 from the same institution. During his PhD he designed and constructed single molecule fluorescence spectroscopy instrumentation in Leeds and applied this to simple studies of protein fol...
Format:PaperbackDimensions:280 pages, 9.69 × 6.73 × 0.01 inPublished:September 1, 2013Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199673845

ISBN - 13:9780199673841

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Table of Contents

1. Introduction2. Single molecule fluorescence techniques3. Single molecule fluorescence instrumentation4. Preparation of samples for single molecule fluorescence spectroscopy5. Fluorescence spectroscopy of freely diffusing single molecules: examples6. Fluorescence spectroscopy of immobilized single molecules: examples7. The outlook for single molecule fluorescence measurements

Editorial Reviews

"An excellent book ... the first text I know of that brings relevant information together in a consistent style. It could end up a classic in the field." --Clive Bagshaw, University of Leicester